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Assessment of sperm function by using confocal laser microscopy

Research Project

Project/Area Number 05671316
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionTottori University

Principal Investigator

MIYAGAWA Ikuo  INSTITUTION,DEPARTMENT,TITLE OF POSITION TOTTORI UNIVERSITY FACULTY OF MEDICINE,PROFESSOR, 医学部, 教授 (60006781)

Co-Investigator(Kenkyū-buntansha) SOFIKITIS Nikolaos  TOTTORI UNIVERSITY FACULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 助手 (60252863)
HIRAKAWA Shinji  INSTITUTION,DEPARTMENT,TITLE OF POSITION TOTTORI UNIVERSITY FACULTY OF MEDICINE,, 医学部, 助教授 (00181149)
戸田 稔子  鳥取大学, 医学部, 助手 (10163990)
山根 明文  鳥取大学, 医学部, 助手 (20174758)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1995: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Keywordsspermatozoom / laser microscopy / fartile potential / acrosin activity / HOST / male infertility / mid-piece
Research Abstract

We have used semen analysis to evaluate sperm function. However, this parameter is not much correlated with fertile potential.
we evaluated sperm morphometric parameters of idopathic infertile men and infertile men with valicocele by using confocal laser scanning microscopy and assested whether these parameters measured by this method were correlated with acrosin activity, HOST (hypoosmotic swelling test) , and fertile potential.
We concluded as follows.
1) The length of sperm mid-piece, ratio of acrosomal region of sperm head, acrosin activity, and HOST is significantly different between fertile men and infertile men.
2) The length of sperm mid-piece and the ratio of acrosomal region of sperm head are much correlated with the percentage of sperm motility and acrosin activity, respectively. Inaddition, the percentage of normal morphometric spermatozoa is much correlated with the fertile potential.
3) Values of sperm morphometric parameters after varicocelectomy can reliably predict sperm fertilizing potential.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Nikolaos V. Sofikitis, M. D., Ph. D. ‡§ Toshiko Toda. M. D., Ph. D. ¶ Ikuo Miyagawa, M. D., Ph.D. ‡ Akihiro Iino, M. D., Ph.D. ^<**> Panaviotis M. Zavos, Ed. S., Ph. D. || Naoki Terakawa, M. D., Ph. D. ¶: "Confocal scanning laser microscopy of morphometric human sperm parameters: correlation with acrosin profiles and fertilizing capacity^*+" FERTILITY AND STERILITY. 62. 376-386 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshisa Yamamoto, Koji Ono. Nikolaos Sofikitis, Ikuo Miyagawa, Yonago. Japan; Fernado Rodriguez, Chapel Bill. NC; Suresh Sikka, Wayne Hellstrom, New Orleansl. AL (Presentation by Dr. Yamamoto).: "SPERM MORPHOMETRY ASSAY HAS HIGH PREDICTIVE VALUES FOR FERTILITY POTENTIAL IN MEN WITH LEFT VARICOCELE (LV) AFTER VARICOCELECTOMY (VRM)." The Journal of UROLOGY. 153. 259- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] NIKOLAOS SOFIKITIS, IKUO MIYAGAWA, DIMITRIOS DIMITRIADIS, PANAYIOTIS ZAVOS, SURESH SIKKA AND WAYNE HELLSTROM: "EFFECTS OF SMOKING ON TESTICULAR FUNCTION, SEMEN QUALITY AND SPERM FERTILIZING GAPACITY" The Journal of UROLOGY. 154. 1030-1034 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nikolaos V.Sorikitis.M.D., Ph. D..<plus-minus>.* Toshiko Toda.M.D., Ph.D.* Ikuo Miyagawa.M.D., Ph.D.* Akihiro Iino.M.D., Ph.D^<****> Panaviotis M.Zavos.Ed.S., Ph.D.|| Naoki Terakawa.M.D., Ph.D.!: "Confocal scanning laser microscopy of morphometric human sperm parameters : correlation with acrosin profiles and fertilizing capacity^* *" FERTITLITY AND STERILITY. Vol.62. 376-386 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yasuhisa Yamamoto, Koji Ono, Nikolaos Sofikitis, Ikuo Miyagawa, Yonago, Japan ; Fernado Rodriguez. Chapel Hill, NC ; Suresh Sikka, Wayne Hellstrom, New Orleans, LA (Presentation by Dr.Yamamoto).: "SPERM MORPHOMETRY ASSAY HAS HIGH PREDICTIVE VALUES FOR FERTILITY POTENTIAL IN MEN WITH LEFT VARICOCELE (LV) AFTER VARICOCELETOMY (VRM)." The Journal of UROLOGY. Vol.153 (APRIL). 259 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] NIKOLAOS SOFIKITIS.IKUO MIYAGAWA.DIMITRIOS DIMITRIADIS.PANAYIOTIS ZAVOS.SURESH SIKKA AND WAYNE HELLSTROM: "EFFECTS OF SMOKING ON TESTICULAR FUNCTION,SEMEN QUALITY AND SPERM FERTILIZING CAPACITY" The Journal of UROLOGY. Vol.154 (September). 1030-1034 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nikolaos V.Sofikitis,M.D.,Ph.D.【double plus】.||,§ Toshiko Toda,M.D.,Ph.D.¶ Ikuo Miyagawa,M.D.,Ph.D.【double plus】 Akihiro Iino,M.D.,Ph,D^<**> Panayiotis M.Zavos.Ed.S.,Ph.D.|| Naoki Terakawa, M.D.,Ph. : "Confocal scanning laser microscopy of morphometric human sperm parameters : correlation with acrosin profiles and fertilizing capacity^★ †" FERTILITY AND STERILITY. 62. 376-386 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yasuhisa Yamamoto,Koji Ono,Nikolaos Sofikitis,Ikuo Miyagawa,Yonago,Japan ; Fernado Rodriguez, Chapel Bill,NC ; Suresh Sikka,Wayne Hellstrom,New Orleans,LA (Presentation by Dr.Yamamoto).: "SPERM MORPHOMETRY ASSAY HAS HIGH PREDICTIVE VALUES FOR FERTILITY POTENTIAL IN MEN WITH LEFT VARICOCELE (LV) AFTER VARICOCELECTOMY (VRM)." The Journal of UROLOGY. 153. 259 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] NIKOLAOS SOFIKITIS,IKUO MIYAGAWA,DIMITRIOS DIMITRIADIS,PANAYIOTIS ZAVOS,SURESH SIKKA AND WAYNE HELLSTROM: "EFFECTS OF SMOKING ON TESTICULAR FUNCTION,SEMEN QUALITY AND SPERM FERTILIZING GAPACITY" The Journal of UROLOGY. 154. 1030-1034 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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