STUDY OF THE ROLES OF PTH RELATED PEPTIDE (PTHrp) IN REPRODUCTION AND GYNECOLOGIC TUMOR
Grant-in-Aid for General Scientific Research (C)
|Allocation Type||Single-year Grants|
Obstetrics and gynecology
|Research Institution||UNIVERSITY OF TOKYO,DEPARTMENT OF MEDICINE,BRANCH HOSPITAL|
KATO Yoshio UNIVERSITY OF TOKYO,DEPT.OF MEDICINE,BRANCH HOSPITAL,ASSISTANT PROFESSOR, 医学部・附属病院(分), 講師 (00185878)
|Project Period (FY)
1993 – 1995
Completed(Fiscal Year 1995)
|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1995 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 1994 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 1993 : ¥700,000 (Direct Cost : ¥700,000)
|Keywords||PTHrP / Ovarian tumor / Cystic fluid / Gene expression / Reproductive organ / Amniotic fluid / Follicular fluid / Human milk|
(1) PTHrP gene expression and presence of PTHrp molecule in normal female reproductive organs.
(a) PTHrP gene expression was confirmed by means of RT-PCR in endometrium, decidua, villi, tube, ovary, and uterine muscle as well as fetal membrane and fetal liver, intestine and skin.
(b) Immunohistochemical analysis showed that PTHrP was positively stained in endometrial cells in endometrium, endometrial cells, stromal cells in decidual tissue, chorionic cells, decidual stromal cells in fetal membrane, syntijtium in chorionic villi.
(c) High concentration of PTHrP was present in milk of lactating puerperal women, in whom serum PTHrP concentration was high, suggesting the transfer of milk PTHrP to the blood stream of lactating puerperal women occured.
(d) PTHrP concentration in amniotic fluid was the highest in second trimester during pregnancy. Amniotic fluid PTHrP concentration in hydroamnios and pregnancies with anomalous fetus was not different from normal controls expect for thanatophoric dwrafism in which PTHrP concentration was lower.
(e) Follicular fluid contained high concentration of PTHrP.PTHrp levels seemed not tobe affected by estrogen or progesterone levels in follicular fluid.
(2) PTHrP gene expression in tissues of ovarian benign and malignant tumors and clinical signific ance of PTHrP in patients with ovarian tumors.
(a) PTHrP gene expression was seen in tissue of both benign and malignant tumors.
(b) PTHrP concentration was determined by means of RIA in sera of patients with benign ovarian tumors (n=34), malignant ovarian tumors (n=27) and ascites and cystic fluid (from benign tumors, n=5, malignant tumors, n=10). High serum levels of PTHrP were seen in 8 % of cases with benign tumors and in 35 % of cases with malignant tumors. Concentration of PTHrP in asctic and cystic fluid was very high (more than 500 pmol/L) in 33 % of malignant cases. These results suggest that PTHrP determination is useful a tumor marker in some cases of ovarian tumors.
Research Output (10results)