GROWTH REGULATION AND MUTATION OF CHROMOSOME TREATED WITH STEROID HORMONS AND ANTICANCER DRUGS ON ENDOMETRIAL CANCER CELLS IN VITRO
Grant-in-Aid for General Scientific Research (C)
|Allocation Type||Single-year Grants|
Obstetrics and gynecology
|Research Institution||IWATE MEDICAL UNIVERSITY|
IZUTSU Toshihiko IWATE MEDICAL UNIVERSITY.DEPARTMENT OF OBSTETRICS AND GYNECOLOGY.ASSOCIATE PROFFSSOR, 医学部, 助教授 (00103739)
FUKUSHIMA Akimune IWATE MEDICAL UNIVERSITY.DEPARTMENT OF OBSTETRICS AND GYNECOLOGY.RESIDENT, 医学部, 助手 (20208937)
SATO Masayuki IWATE MEDICAL UNIVERSITY.DEPARTMENT OF OBSTETRICS AND GYNECOLOGY.RESIDENT, 医学部, 助手 (20183387)
|Project Period (FY)
1992 – 1994
Completed(Fiscal Year 1994)
|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1994 : ¥800,000 (Direct Cost : ¥800,000)
Fiscal Year 1993 : ¥1,300,000 (Direct Cost : ¥1,300,000)
|Keywords||ENDOMETRIAL CANCER / CHROMOSOME / STEROID HORMONE / supressor gene / oncogene|
I) Chromosomal analysis of endometrial cancer cells treated with steroid hormon
Endometrial cancer cell line (Ishikawa, HEC-1) were treated with 2x10^<-5>M Progesterone (P) and 2x10^<-5>M Danazol (D).
Ishikawa (IK) cell was not decreased treated with p or D.
HEC-1 (HEC) cell also was not decreased with P and D.
No chromosomal abnormalities were observed treated with Por D in IK or HEC.
II) chromosomal analysis of endometrial cancer cells treated with anticancer drug
Endometrial cancer cell line (IK) were treated with 5mug/ml CDDP (C) or 1x10^<-5>M Taxol (T).
Growth suppression (74%) was observed in IK cell after 24 hours exposure with C.
Growth suppression (62%) was observed in IK cell after 24 hours exposure with T.
HEC cell was not decreased after 4,8, or 24 hours exposure with C.
Growth suuppression (57%) was observed in IK cell after 4 hours exposure with T.
No chromosomal abnormalities were observed treated with T in IK or HEC.
chromosomal abnormalities such as gap, ctb, cte and csb were observed treated with C in IK or HEC.
Research Products (25results)