|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1994 : ¥900,000 (Direct Cost : ¥900,000)
Fiscal Year 1993 : ¥1,200,000 (Direct Cost : ¥1,200,000)
It has been reported that a soluble lacrimal gland antigen (LG-Ag) was capable of inducing experimental autoimmune dacryoadenitis (EAD) in SJL/J mice. (S.H.Liu, et al, Invest.Ophthalmol Vis Sci, 33 : 2029.1992) This animal model is thought to be a good model in the study of dry eye syndrome including Sjogren syndrome. In this study we analyzed the lymphocyte subsets of infiltrating cells, especially T cells, in the lacrimal gland of EAD.
We immunized ten female SJL/J mice with LG-Ag (100mug) in complete Freund's adjuvant (CFA). Thirty and fourty days after immunization, the exorbital lacrimal glands were removed and fixed in liquid nitrogen. Five micron cryostat sections were stained by ABC technique of immunoperoxidase methods. The following monoclonal antibodies were used : 1) anti-Thy-1,2) anti-L3T4,3) anti-Lyt-2.
Histologically many mononuclear inflammatory cells were observed mainly around the lacrimal ducts with considerable destruction of LG acini and ducts. Immunohistochemically many Thy-1 positive lymphocytes were seen in the area or infiltrating cells. L3T4 of helper T cells seemed to be a predominant population of T cells whereas Lyt-2 of suppressor/cytotoxic T cells were a minor population of T cells.
The above findings indicate that T cells, especially helper T cells, may play a role in the immunopathology of EAD.