| Project/Area Number |
05671922
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
AKAMIZU Takashi Kyoto Univ., Dept.of Labo.Med.Assist.Prof., 医学部, 助手 (20231813)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Fumihiko Kyoto Univ., Cent.for Mol.Biol.&Genetics, Assist.Prof., 遺伝子実験施設, 助手 (50212220)
SUGAWA Hideo Kyoto Univ., Dept.of Labo.Med.Lecturer, 医学部, 講師 (70162857)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | immunoglobulin genes / Anti-TSH receptor antibodies / TSH receptor, / Thyrotropin / Graves'disease, / Hypothyroidism / Myxedema / Autoantibodies |
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| Research Abstract |
To elucidate the etiological aspects of autoimmune diseases, we aimed to study immunogloblin genes encoding autoantibodies, anti-TSH receptor antibodies. We prepared EB virus transformed B lymphocytes producing anti-TSH receptor antibodies from peripheral blood lymphocytes of patients with Graves' disease and primary hypothyroidism. Variable regions of immunoglobulin genes of these lymphocytes were isolated and sequenced. We obtained following findings :
1) Repertoires of immunoglobulin variable genes were investigated and their restricted usage were found both in heavy and light chains.
2) Sequence analysis revealed that a number of replacement (R) and silent (S) somatic mutations were found both in CDR and framework and the R/S ratios of CDR tended to be higher than those of the framework, suggesting the importance of somatic mutations for antibodies activities.
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