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Improvement of Culture Method and Elucidation of Pahogenic Factors of CAR Bacillus

Research Project

Project/Area Number05680745
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Laboratory animal science
Research InstitutionCentral Institute for Experimental Animals (CIEA)

Principal Investigator

ITOH Toshio  CIEA, Laboratory Animal Medicine, Chief, 動物医学研究室, 室長 (20106644)

Co-Investigator(Kenkyū-buntansha) OHASHI Hiroaki  CIEA, Laboratory Animal Medicine, Researchier, 動物医学研究室, 研究員 (30250012)
Project Period (FY) 1993 – 1995
Project Status Completed(Fiscal Year 1995)
Budget Amount *help
¥1,900,000 (Direct Cost : ¥1,900,000)
Fiscal Year 1995 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 1994 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 1993 : ¥700,000 (Direct Cost : ¥700,000)
KeywordsCAR bacillus / Improvement of culture method / Pathogenesis / カ-バチルス / 実験動物 / 培養方法 / 感染病 / マウス / 細菌
Research Abstract

Cilia-associated respiratory (CAR) bacillus is an unique respiratory pathogen for laboratory animals such as mice, rats and rabbits, which is difficult to culture. Because of the difficulty of propagation of this organism, there are few studies concerning its pathogenesis. The purposes of this study were improvement of the culture methods and elucidation of pathogenic factors.
For improvement of the culture methods, embryonated chicken eggs and in vitro cultures were studied. Embryonated chicken egg-associated CAR bacillus was not established by passaging. Changes in the components of the culture medium and the use of a culture bottle courted with chemical substances did not affect growth.
Concerning the pathogenicity of this organism, repeated administration of a large number of killed organisms did not produce any lung lesions in mice. Because we failed to improve cultures of this organism, and could not obtain sufficient amounts of CAR bacillus, studies on pathogenicity were inadequate.

Report

(4results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report

Research Products

(3results)

All Other

All Publications

  • [Publications] Kazuo Goto: "Detection of cilia-associated respiratory bacillus in experimentally and naturally infected mice and rats by the polymerase chain reaction"Experimental Animals. 44. 333-336 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kazuo Goto, Ryoko Nozu, Akira Takakura, Satoru Matsushita and Toshio Itoh: "Detection of cilia-associated respiratory bacillus in experimentally and natrually infected mice and rats by the polymerase chain reaction"Exp. Anim.. 44(4). 333-336 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kazuo Goto: "Detection of Cilia-Associated Respiratory Bacillus in Experimentally and Naturally Infected Mice and Rats by the Polymerase Chain Reaction" Exp.Anim.44. 333-336 (1995)

    • Related Report
      1995 Annual Research Report

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Published : 1993-04-01   Modified : 2016-04-21  

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