TOJO Naoko Tokyo Medical & Dental University, Instructor, 医学部, 助手 (90227554)
TOMITA Kimio Kumamoto Univ., Medical School, Professor, 医学部, 教授 (40114772)
MARUMO Fumiaki Tokyo Medical & Dental University, Professor, 医学部, 教授 (00050443)
|Budget Amount *help
¥1,700,000 (Direct Cost : ¥1,700,000)
Fiscal Year 1994 : ¥900,000 (Direct Cost : ¥900,000)
Fiscal Year 1993 : ¥800,000 (Direct Cost : ¥800,000)
Renal nephron segments were heterogeneous, and receptors for endothelin (ET)-1, ET-3, Angiotensin II (AT II), EGF,and IGF-1 distribute differently along the nephron segments. We have revealed that the PCR localizatoin of two types (V2, V1) of vasopressin receptor, and angiotensin II receptor. We also reported that localization of mRNA and protein of ET-3 along the nephron segments. Recently, growth factors and vasoactive substances are reported to stimulate mitogen-activated protein kinase (MAP-K). In this study, we showed that mRNA of MEK-K,Raf-1-K,MAPK-K,MAP-Ks(p42 and p44), and S6-K was expressed in nephron segments using RT-PCR technique. We showed that MAP-K cascades are expressed in every nephron segment. ET-1, ET-3, AT II,EGF,and IGF-I stimulate MAP-K cascades heterogeneously along the nephron segment. We concluded that MAP-K cascades play an important role in the regulation of renal function. We also reported that the presence of C-type natriuretic factor and its receptor (GC-B) along the nephron. The CNP system may play an important roles in modulatiing the salt and body fluid homeostasis and blood pressure. We also analyzed the expression of PDGF and PDGF-receptor expression in the glomeruli of IgA nephropathy patients. Thus, we investigated the new approaches to the pathogenesis of glomerulonephritis and renal functions.