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Environmental adaptation of Echinococcus in South America

Research Project

Project/Area Number 06041005
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionField Research
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

OKU Yuzaburo  Hokkaido University, Graduate School of Veterinary Medicine, Associate Professor, 大学院獣医学研究科, 助教授 (60133716)

Co-Investigator(Kenkyū-buntansha) AROTCE Pedro  University of Republic of Uruguay, Institute of Hygiene, Instructor, 衛生研究所, 助手
BENAVIDEZ Ur  ウルグアイ共和国大学, 衛生研究所, 助手
MALGOR Ramiro  University of Republic of Uruguay, Institute of Hygiene, Instructor, 衛生研究所, 助手
IWAKI Takashi  Tottori University, Faculty of Medicine, Technical official, 医学部, 教務職員 (70263473)
OKAMOTO Munehiro  Osaka University, Medical School, Instructor, 医学部, 助手 (70177096)
KAMIYA Masao  Hokkaido University, Graduate School of Veterinary Medicine, Professor, 大学院獣医学研究科, 教授 (30081665)
BENAVIDEZ Uruguaysito  University of Republic of Uruguay, Institute of Hygiene, Instructor
YARZABAL Lui  ウルグアイ共和国大学, 衛生研究所, 所長
VENAVIDEZ Ur  ウルグアイ共和国大学, 衛生研究所, 助手
八木 欣平  北海道立衛生研究所, 研究員
野中 成晃  北海道大学, 獣医学部, 学術振興会特別研究員
OOI Hong Kea  北海道大学, 獣医学部, 助手 (40223440)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥15,700,000 (Direct Cost: ¥15,700,000)
Fiscal Year 1995: ¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1994: ¥8,500,000 (Direct Cost: ¥8,500,000)
KeywordsEcinococcus granulosus / Uruguay / Genetic variation / Coproantigen / Live stock / Alternativa hosts / Epidemiology / Environmental adaptation / 南米 / 感染圧
Research Abstract

Epidemiological status and environmental adaptation of Echinococcus granulosus in Uruguay were studied. Most important industry of Urugyay is livestock industry. A questionnaire survey shown that about 20% of the inhabitants carried out home-slaughtering of sheep. Dogs have access to the viscera of sheep in farms.
Viscera of 639 sheep were sliced and examined for hydatid cysts. Prevalence of hydatid cysts in sheep was about 40%. and mean intensity of hydatid cysts in examined sheep was 1.67. Most sheep harbored few cysts. Prevalence of hydatid disease in sleep increased with age. Half of adult sheep had hydatid cyst. Average increase in number of cysts per year was about 1. More than 3 years old sheep harbored fertile cysts, Prevalence in more than 5 years old sheep was about 10%. Mean number of fertile cysts in more than 5 years old sheep was 4. The mean number of protoscoleces per fertile cyst is 1,437. Most of the fertile cysts contain less than 500 protoscoleces.
We examined the prevalence in dogs by arecoline survey. Prevalence of E.granulosus in farms is about 23% or more. Prevalence in dogs in town is lower than that in farm. In a survey by necropsy of stray dogs in town, 3 of 77 dogs were infected.
DNA sequence of COI of E.granulosus from sheep and cattle in Uruguay were analyzed for determinination of genetic variation. The results showed that common sheep strain distribute in Uruguay.
We developed a sandwich ELISA using monoclonal antibody for detection of coproantigen in E.granulosus infected dogs. The time course of coproantigen excretion from dogs experimentally infected with E.granulosus was monitored by this ELISA.
Tapeworm stage of E.granulosus developed in the small intestine of immunosuppressed jirds, but fully matured parasite were not recovered from these animals.

Report

(2 results)
  • 1995 Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] IWAKI,T.: "Infectivity to rats with eggs of the Echinococcus multilocularis isolate from a Norway rat in Hokkaido,Japan." Japanese Journal of Parasitology. 44. 32-33 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] IWAKI,T.: "New distribution record of Taenia mustelae Gmelin,1790 (Cestoda) from the least weasel Mustela nivalis in Hokkaido" Japanese Journal of Parasitology. 44. 796 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] KOHNO,H.: "Development and characterization of murine monoclonal antibodies to Echinococcus multilocularis adult worms and its use for coproantigen detection." Japanese Journal of Parasitology. 44. 401-412 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] SAKASHITA,M.: "Detection of Echinococcus multilocularis coproantigens in experimentally infected dogs using murine monoclonal antibody against adult worms" Japanese Journal of Parasitology. 44. 413-420 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] SAKAI,H.: "An enzyme-linked immunosorbent assay (ELISA) for the detection of Echinococcus granulosus coproantigens in dogs." Japanese Journal of Parasitology. 44. 453-461 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] KAKUDA,T.: "Participation of platelets in protection against larval Taenia taeniaeformis infection in mice" International Archives of Allergy and Immunology. 109. 295-297 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] OKAMOTO,M.: "Phylogenetic relationships within Taenia taeniaseformis variants and other taeniid cestodes inferred from the nucleotide sequence of the cytichromec oxidase subunit I gene." Prasitology Research. 81. 451-458 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] MALGOR,R.: "High prevalence of Ancylostoma spp.infection in dogs in Tacuarembo,Uruguay." Parasite. 3(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] INOHARA,J.: "Acquired resistance against adult Echinococcus multilocularis in fection observed in golden hamsters" Japanese Journal of Parasitology. 45(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] SAKAI,H.: "Echinococcus multilocularis coproantigen detection in golden hamster,an alternative definitive host" Experimental Animals. 45(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] INOHARA,J.: "Parenteral strobilar development of Echinococcus multilocularis in SCID mice" Japanese Journal of Veterinary Research. 44(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] IWAKI,T.: "Infectivity to rats with eggs of the Echinococcus multilocularis isolate from a Norway rat in Hokkaido, Japan." Japanese Journal of Parasitology. 44. 32-33 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] IWAKI,T.: "New distribution record of Taenia mustelae Gmelin, 1790(Cestoda) from the least weasel Mustela nivalis in Hokkaido" Japanese Journal of Parasitology. 44. 796 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] KOHNO,H.: "Development and characterization of murine monoclonal antibodies to Echinococcus multilocularis adult worms and its use for the coproantigen detection." Japanese Journal of Parasitology. 44. 401-412 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] SAKASHITA,M.: "Detection of Echinococcus multilocularis coproantigens in experimentally infected dogs using murine monoclonal antibodv against adult worms" Japanese Journal of Parasitology. 44. 413-420 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] SAKAI,H.: "An enzyme-linked immunosorbent assay (ELISA) for the detection of Echinococcus granulosus coproantigens in dogs." Japanese Journal of Parasitology. 44. 453-461 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] OKAMOTO,M.: "Phylogenetic relationships within Taenia taeniaseformis variants and other taeniid cestodes inferred from the nucleotide sequence of the cytichrome c oxidase subunit I gene." Prasitology Research. 81. 451-458 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] KAKUDA,T.: "Participation of platelets in protection against larval Taenia taeniaeformis infection in mice" International Archives of Allergy and Immunology. 109. 295-297 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] SAKAI,H.: "Echinococcus multilocularis coproantigen detection in golden hamster, an alternative definitive host" Experimental Animals. 45(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] MALGOR,R.: "High prevalence of Ancylostoma spp. infection in dogs in Tacuarembo, Uruguay." Parasite. 3(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] INOHARA,J.: "Acquired resistance against adult Echinococcus multilocularis in fection observed in golden hamsters" Japanese Journal of Parasitology. 45(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] INOHARA,J.: "Parenteral strobilar development of Echinococcus multilocularis in SCID mice" Japanese Journal of Veterinary Research. 44(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] IWAKI,T: "Developmental and morphological characteristics of Taenia taeniaeformis(Batsh,1786)in Clethrionomys ruiocanus bediordiae and Ratcus norvegicus from different geographical locations." J.Parasitol..80. 461-467 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] NONAKA,N.: "Infectivities of four isolates of Taenia taeniaeformis to various rodents." J.Vet.Med.Sci..56. 565-567 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] AZUMA,H..: "Intraspecific variation of Taenia taeniaeformis as determined by various criteria." Parasitol.Res.81. 103-108 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] OKAMOTO,M..: "Applocation of DNA fingerprinting with digoxigenated oligonucleotide probe(CAC)5 to analysis of the genetic variation within Taenia taeniaefrormis" J.Vet.Med.Sci.57. 267-272 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] OKAMOTO,M.: "Intraspecific variation of isoenzymes in Taenia taeniaformis." Int.J.Parasitol..25. 221-228 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 奥 祐三郎: "多包条虫と単包条虫の比較生物学とその対策" 家畜衛生技術協議会集録. 17号. 16 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] OKAMOTO,M.: "Phylogenetic relationships within Taenia taeniaseformis variants and other taeniid cestodes inferred from the nucleotide sequence of the cytichrome c oxidase subunit I gene." Prasitol.Res.81(in press). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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