| Project/Area Number |
06044082
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Niigata University |
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Principal Investigator |
HOSHINO Etsuro School of Dentistry, Niigata Univ., Professor, 歯学部, 教授 (90124619)
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| Co-Investigator(Kenkyū-buntansha) |
GREENMAN John Univ.of the West of England, Senior Lecturer, Univ. of the, Senior Lec
WEIGHTMAN Andrew j. Univ.of Wales College of Cardiff, Lecturer, Univ. of, Lecturer
ELWORTHY Alison j. Univ.of the West of England, Research Associate, Univ. of the, Research A
HIOM Sarah j. Univ.of Bristol, Research Associate, Univ. of Bristol, Research A
WADE William g. Univ.of Bristol, Reader, Univ. of Bristol, Reder
NAKAZAWA Futoshi Niigata Univ., Lecturer, 歯学部, 講師 (60115053)
SATO Takuichi JSPS, Reserch Fellow, 特別研究員
HIOM Salah ブリストル大学, 歯学部, 助手
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1995: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1994: ¥3,200,000 (Direct Cost: ¥3,200,000)
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| Keywords | Eubacterium / asaccharolytic / oral cavity / obligate anaerobes / taxonomy / new species |
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| Research Abstract |
Asaccharolytic Eubacterium species are strictly anaerobic, gram-positive rods and are frequently isolated and predominant bacterial species from different oral samples. The slow growth and oxygen sensitivity of the oral asaccharolytic Eubacterium species has hindered the determination of their significance in various oral infectious diseases. By adopting efficient anaerobic isolation procedures, we isolated several unclassified strains of Eubacterium that were distinct from the established Eubacterium species namely, E.brachy, E.lentum, E.nodatum and E.timidum. A polyphasic approach was used to examine the taxonomic positions of these organisms. These un-named strains were inert in conventional biochemical tests, however, the results of SDS-PAGE protein profile analysis and aminopeptidase activty (Rapid ID 32A) revealed that these strains were quite distinct compared from the established asaccharolytic species and assigned to four groups. Two groups of strains, is butyrate producing gram-positive short rods, have been proposed new species namely, E.saphenum and E.minutum, established on the additional basis of western immunoblotting reactions and DNA-DNA hybridization analysis.The third group were phenylacetate producing strains, which can be distinguished from E.timidum by the differences in whole-cell protein profile and aminopeptidase activity. The strains in the fourth group produced a trace (if any) of phenylacetate. The continuos development and improvement of anaerobic incubation and identification techniques resulted in the isolation of slow growing and culture-difficult asaccharolytic species of Eubacterium. Further studies are in progress to provide information on the roles of the unclassified strains and other Eubacterium species in oral disease.
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