Studies on molecular basis of pathogenesis of rinderpest virus and molecular epidemiology
Grant-in-Aid for international Scientific Research
|Allocation Type||Single-year Grants|
|Research Institution||Nippon Institute for Biological Science|
YAMANOUCHI Kazuya Nippon Institute for Biological Science, Chief Researcher, 主任研究員 (30072888)
KOBUNE Fumio National Institute of Health, 主任研究員 (80142644)
NUNOYA Tetsuo Nippon Institute for Biological Science, 主任研究員 (80099994)
SUGIYAMA Makoto Gifu University, 農学部, 助手 (80196774)
KAMATA Hiroshi Nihon University, 農獣医学部, 講師 (30139050)
KAI Chieko University of Tokyo, 助教授 (10167330)
AMADA Deiaro フランス熱帯獣医学研究所, 主任研究員
THOMAS Barre 英国家畜衛生研究所, 主任研究員
BARRETT Thomas BBSRC Institute for Animal Health, UK
DIALLO Adama Institute d'Elevage et de Medecine Veterinaire des pays Tropicaux, France
DIALLO Adama フランス熱帯獣医学研究所, 主任研究員
BARRETT Thom 英国家畜衛生研究所, 主任研究員
|Project Period (FY)
Completed(Fiscal Year 1995)
|Budget Amount *help
¥12,200,000 (Direct Cost : ¥12,200,000)
Fiscal Year 1995 : ¥5,800,000 (Direct Cost : ¥5,800,000)
Fiscal Year 1994 : ¥6,400,000 (Direct Cost : ¥6,400,000)
|Keywords||Rinderpest virus / Pathogenesis / Molecular epidemiology / Recombinant vaccine / Genetic analysis / PPR virus / Molecular biology / Celluar immunity|
The aim of the collaborative research on rinderpest virus was to conduct basic virological and immunological studies and to apply the results on the molecular diagnosis, epidemiological survey and control of rinderpest in endemic areas.The Japanese exertise in recombinant rinderpest vaccine and pathogenesis in rabbit model of rinderpest, the expertise and facilities of UK group as an international center of rinderpest, and the expertise in peste des petits ruminants (PPR) in French group made highly effective collaborative research team.
Major results obtained are briefly summarized as below.
1.Virological and immunological studies on pathogenesis of rinderpest in rabbit model and its natural host, cattle
Primary target of rinderpest virus in rabbits was identified as CD5+T cells followed by wide spread in lymphoid tissues affecting most of the lymphoid cells.
Quantitative determination in lymphoid tissues of cattle became possible by the use of B95a system, and the results were compared with those in rabbits.
2.Studies on recombinant rinderpest vaccine based on vaccinia virus vector The efficancy, duration of immunity, safety and genetic stability of the recombinant rinderpest vaccine in cattle were found to be satisfactory.Considering its high heat stability and easy differentiation from natural infection, practical application for the control of rinderpest was clearly indicated through these studies.
3.Molecular epidemiological studies
A panel of monoclonal antibodies to rinderpest virus H protein was developed, and applied for differential diagnosis between rinderpest and PPR.
Research Output (10results)