Grant-in-Aid for Scientific Research (A)
|Allocation Type||Single-year Grants|
|Research Institution||Tohoku University|
FUJIMURA Shigefumi Institute of Development, Aging and Cancer, Tohoku University Professor, 加齢医学研究所, 教授 (40006078)
SHIRATO Kunio First Department of Internal Medicine, Tohoku University Professor, 医学部, 教授 (80004761)
KOYANAGI Hitosi Department of Cardiovascular Surgery, Tokyo Women's Medical Colledge Professor, 医科大学, 教授 (90138884)
MATSUDA Hikaru First Department of Surgery, Osaka University Professor, 医学部, 教授 (00028614)
FURUSE Akira Department of Cardiothoracic Surgery, Tokyo University Professor, 医学部, 教授 (70010163)
KATO Harufumi Department of Surgery, Tokyo Medical Colledge Professor, 教授 (20074768)
小松 作蔵 札幌医科大学, 教授 (00045329)
富田 正雄 長崎大学, 医学部, 教授 (70039808)
|Project Period (FY)
1994 – 1996
Completed(Fiscal Year 1996)
|Budget Amount *help
¥30,100,000 (Direct Cost : ¥30,100,000)
Fiscal Year 1996 : ¥4,900,000 (Direct Cost : ¥4,900,000)
Fiscal Year 1995 : ¥11,300,000 (Direct Cost : ¥11,300,000)
Fiscal Year 1994 : ¥13,900,000 (Direct Cost : ¥13,900,000)
|Keywords||Lung Transplantation / Heart-Lung Transplantation / Pulmonary Preservation / Immunosuppression / Rejection Monitoring / Non-Heart-Beating Donor|
1.Preservation : It was demonstrated that extracellular composition with buffer action of preservation solution and temperature were both quite important factors in preservation. Nitric oxide inhalation proved to improve the function of preserved graft. To evaluate the function of preserved graft, measurement of filtration coefficient or pulmonary alveolar fluid clearance were likely to be useful.
2.Reimplantation response : Urinastatin, g-hydroxybutylate, thioredoxin, a-blocker were found to have protective effects on posttransplantation pulmonry edema, suggesting that not only the reperfusion injury but also the autonomic denervation participated in this phenomenon.
3.Rejection and immunosuppression : Cytotoxicity test against donor skin fibroblast were useful method to detect (100) acute rejection in canine lung transplantation, and the extent of the mononuclear cell infiltration in pulmonary allograft correlated to the value of cytotoxicity index. To monitor rejection in lung transpl
antation, measurement of nitric oxide gas concentration is likely to be useful since the increase in the concentration of that was correlated with the pathological grading of rejection.
4.Expansion of the donor source : Lung transplantation from the brain-dead, non-heart-beating donor was demonstrated to be possible in the primate sequential bilateral lung transplantation model. Lung transplantation from non-heart-beating donor will expand the indication of the donor criteria, resulting in the increase of donor source. In the field of xenotransplantation, the possibility to induce the tolerance in concordant heart transplantation model between mouse and rat was shown with the administration of anti-T-cell receptor antibody.
5.Recipient criteria : To determine the appropriate point for transplantstion, the term from the starting point of oxygen inhalation to death was evaluated in 14 cases with diffuse pulmonary diseases. Although further (200) experience is necessary, it was postulated that the starting point of oxygen supplement was appropriate for the registration since most cases had died within 3 years from that point. Less