| Project/Area Number |
06304053
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | National Institute of Neuroscience, NCNP |
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Principal Investigator |
NABESHIMA Yo-ichi Dep.of Mol.Genetics, Natl.Inst.of Neuroscience Director, 神経研究所・遺伝子工学, 部長 (60108024)
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| Co-Investigator(Kenkyū-buntansha) |
ENDO Takeshi Dep.of Biology Faculty of Science Chiba University Associate, 理学部, 助教授 (30194038)
NOJI Sumihare Dep.of Biological Science and Technology Faculty of Engineering Tokushima Univer, 工学部, 教授 (40156211)
MAKABE Kazuhiro Dep.of Biology Faculty of Science Kyoto University Assistant, 大学院・理学研究科, 助手 (60222288)
OBINATA Takashi Dep.of Biology Faculty of Science Chiba University Professor, 理学部, 教授 (40012413)
ASASHIMA Makoto Dep.of Biology, Tokyo University Professor, 教養学部, 教授 (00090564)
佐藤 矩行 京都大学, 理学部, 助教授 (30025481)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥18,100,000 (Direct Cost: ¥18,100,000)
Fiscal Year 1995: ¥8,200,000 (Direct Cost: ¥8,200,000)
Fiscal Year 1994: ¥9,900,000 (Direct Cost: ¥9,900,000)
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| Keywords | Myogenic Factors / Myogenin / Muscle development / Gene Targeting / Cell differentiation / Growth factor / Cell culture / bHLH / 筋分化 / アポトーシス / 形態形成 / MyoD family / アクチビン / 筋管形成 |
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| Research Abstract |
From the co-operative research on the mechanisms controlling the determination and differentiation of muscle lineage cells, the followings were concluded.
1) Myogenin is critical for muscle cell differentiation from myoblasts to myotubes and for the maturation of muscle fibers. MRF4 is expressed rather intensively in residual myotubes of mutant embryos than in myotubes of wild type littermates. Majority of myogenin-/-myotubes degrade at late embryonic stage. Secondary myotubes are scarcely generated. However, some secondary myoblasts are seen at muscle forming regions.
2) The gene cascade required for the induction of muscle lineage cells from animal caps by the addition of actibin, was analyzed and cis regulatory elements regulating the initiation of myf-5 gene expression in early xenopus embryos, was detected.
3) FGF,Shh, BMP,HGF,and Wn't are involved in the induction of muscle cells in chick myotomes and limb buds and the ectopic expression of FGF in lateral mesoderm induces extra-limb.
4) Expression pattern of proteins regulating the polymerization of actin during embryogenesis and cytokinasis, was analyzed and revealed the importance of phosphorilation of cofilin.
5) A novel G-protein expressed in skeletal muscle cells was identified.
6) All actin genes were isolated from ascidian and examined their expressions in early embryos. Interestingly, some actin genes begin to express prior to the initial expression of MyoD.
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