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OSMOREGULATORY MECHANISMS DURING EARLY LIFE STAGES IN FISH AND THEIR HORMONAL CONTROL

Research Project

Project/Area Number 06405004
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionUNIVERSITY OF TOKYO

Principal Investigator

HIRANO Tetsuya  UNIV.TOKYO,OCEAN RES.INST., PROF., 海洋研究所, 教授 (70013571)

Co-Investigator(Kenkyū-buntansha) TAGAWA Masatomo  UNIV.of TOKYO,OCEAN RES.INST., ASSIST.PROF., 海洋研究所, 助手 (20226947)
KANEKO Toyoji  UNIV.of TOKYO,OCEAN RES.INST., ASSIST.PROF., 海洋研究所, 助手 (70221190)
TANAKA Masaru  KYOTO UNIV., FAC.OF.AGR., PROF., 農学部, 教授 (20155170)
AIDA Katsumi  UNIV.of TOKYO,FAC.OF AGR., PROF., 農学部, 教授 (50012034)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥16,700,000 (Direct Cost: ¥16,700,000)
Fiscal Year 1995: ¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1994: ¥9,000,000 (Direct Cost: ¥9,000,000)
KeywordsEuryhaline fish / Embryos and larvae / Early development / Osmoregulation / Hormones / Cortisol / Prolactin / Chloride cells
Research Abstract

The aim of this study is to clarify osmoregulatory mechanisms during early life stages of euryhaline fishes, specifically in euryhaline tilapia (Oreochromis mossambicus), and their hormonal control. The results obtained during the last 2 years are as follows.
1. Prolactin cells identified by immunocytochemistry and in situ hybridization were developed earlier in the tilapia embryos developed in fresh water (FW) than those in seawater (SW), suggesting its important role in FW adaptation as in adult fish.
2. Chloride cells on the yolk-sac membrane of tilapia embryos and larvae reared in FW developed earlier than in those in SW,and cortisol, SW-adapting hormone in the adult fish, accelerated their development.
3. Tilapia larvae started drinking seawater 2 days after hatching. Drinking rate, as measured by accumulation of ^<14>C-dextran in the gut, was still greater in the larvae in SW than those in FW,and the drinkig rate increased as the larvae grow.
4. Turnover rate of tritiated water in tilapia embryos and larvae in FW were twice as great as those in SW,whereas the turnover rate of ^<36>Cl was 50-80 folds greater in SW than in FW.
5. Field studies indicated the importance of thyroid hormones and cortisol in the development of digestive system during early embryonic stages and also during metamorphosis from larval to juvenile stages in several marine species such as flounders, tunas and seabreams.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Ayson,F.G.et al.: "Differential expression of two prolactin and growth hormone genes during early development of tilapia in freshwater and seawater." General and Comparative Endocrinology. 95. 143-152 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ayson,F.G.et al.: "Development of mitochondrion-rich cells in the yorl-sac membrane of embryos and larvae of tilapia in freshwater and seawater." Journal Experimental Zoology. 270. 129-135 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tagawa,M.et al.: "Thyroid hormone concentrations in the gonads of wild chum salmon during maturation." Fish Physiology and Biochemistry. 13. 233-240 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kaneko,T.et al.: "Hypoosmoregulatory ability of eyed-stage embryos of chum salmon." Marine Biology. 122. 165-170 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ayson,F.G.et al.: "Cortisol stimulates the size and number of mitochondrion-rich csells in the yolk-sac membrane of embyos and larvae of tilapis." Journal Experimental Zoology. 272. 419-425 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tanaka,M.et al.: "Development of the pituitary, thyroid and interrenal glands and applications of endocrinology to the improved rearing of marine fish." Aguaculture. 35. 111-126 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Clarke,W.C.and T.Hirano: "Phys:ological Ecology of Pacific Salmon" University of British Columbia Press, 503 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tanaka,M.: "Survival Strategies at Early Stage of Marine Resources" Balkema, 367 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ayson, F.G., T.kaneko, S.Hasegawa, and T.Hirano: "Differential expression of two prolactin and growth hormone genes during early development of tilapia (Oreochromis mossambicus) in fresh water and seawater : Implications for possible involvement in osmoregulation during early life stages." Gen.Comp.Endocrinol. 95. 143-152 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ayson, F.G., T.Kaneko, S.Hasegawa, and t.Hirano: "Development of mitochondrion-rich cells in the yolk-sac membrane of embryos and larvae of tilapia, Oreochromis mossambicus, in fresh water and seawater." J.Exp.Zool.270. 129-135 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tagawa, M., T.Ogasawara, T.Sakamoto, T.Miura, K.Yamauchi, and T.Hirano: "Thyroid hormone concentrations in the gonads of wild chum salmon during maturation." Fish Physiol.Biochem.13. 233-240 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] de Jesus, E.G., T.Hirano, and Y.Inui: "The antimetamorphic effect of prolactin in the Japanese flounder." Gen.Comp.Endocrinol.93. 44-50 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Inui, Y., S.Miwa, K.Yamano, and T.Hirano: "Hormonal control of flounder metamorphosis. In "Perspectives in Comparative Endocrinology" (K.G.Davey, R.E.Peter, and S.S.Tobe, eds)" 408-411 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kaneko, T., S.Hasegawa, Y.Takagi, M.Tagawa, and T.Hirano: "Hypoosmoregulatroy ability of eyed-stage embryos of chum salmon." Marine Biol.122. 165-170 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ayson F.G., T.Kaneko, S.Hasegawa, and T.Hirano: "Cortisol stimulates the size and number of mitochondrion-rich cells in the yolk-sac membrane of embryos and larvae of tilapia (Oreochromis mossambicus) in vitro and in vivo." J.Exp.Zool.272. 419-425 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tanaka, M., J.B.Tanangonan, M.Tagawa, E.G.de Jesus, H.Nishida, M.Isaka, R.Kimura, and T.Hirano: "Development of the pituitary, thyroid and interrenal glands and applications of endocrinology to the improved rearing of marine fish larvae." Aquaculture. 135. 111-126 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kinoshita, I., S.Fujita, I.Takahashi, K.Azuma, T.Noichi, and M.Tanaka: "A morphological and meristic comparison of larval and juvenile temperate bass, Lateolabrax japonicus, from various sites in western and central Japan." Japan.J.Ichthyol.42. 165-171 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kaneko,T.et al.: "Hypoosmoregulatory ability of eyed-stage embryos of chum salmon." Marine Biolgy. 122. 165-170 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ayson,F.G.et al.: "Cortisol stimulates the size and number of mitochon-drion-rich cells in the yolk sac membrane" Experimental zoology. 272. 419-425 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tanaka,M.et al.: "Development of pituitary,thyroid and interrenal glands and applications of endocrinology" Aquaculture. 135. 111-126 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kinosita,I.et al.: "A morphological and merstic comparison of larval and juvenile temperate bass,from various sites" Japan.Journal of Ichthyology. 42. 165-171 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Makino,N.et al.: "Differentiation and evelopment of the swimbladder in larvae of the Japanese sea bass." Nippon Suisan Gakkaishi. 61. 143-150 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Uchida,K.et al.: "Changes in chloride cell activity in the gill filaments and lamellae during seawater adaptation" Journal Experimental Biology. (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Clarke,W.C.: "physiological Ecology of pacific Salmon" University of British Columbia Press, 503 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tanaka,M.: "Survival Strategies at Early Stages of Marine Resources" Balkema, 367 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ayson,F.G.et al.: "Differentional expression of two prolactin and qrowth hormone qene during early development of tilapia" General and Comparative Endocrinology. 95. 143-152 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ayson,F.G.et al.: "Development of mitochondrion-rich cells in yolk-sac membrane of tilapia larvae in freshwater and seawater" Jounal Experimental Zoology. 270. 129-135 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tagawa,M.et al.: "Thyroid hormone concentrations in the gonad of wild chum salmon during maturation" Fish Physiology and Biochemistry. 13. 233-240 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] de Jesus,E.G.et al.: "The anti-metamorphic effect of prolactin in the Jaoanese flounder" Genral and Comparative Endocrinology. 93. 44-50 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Inui,Y.et al.: "Hormonal control of flounder metamorphosis." Perspectives on Comparative Endocrinology. 408-411 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kaneko,T.er al.: "Hypoosmoregulatory ability of eyed stage embryos of chum salmon,Oncorhynchus keta" Marine Biology. (印刷中).

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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