|Budget Amount *help
¥4,900,000 (Direct Cost : ¥4,900,000)
Fiscal Year 1995 : ¥900,000 (Direct Cost : ¥900,000)
Fiscal Year 1994 : ¥4,000,000 (Direct Cost : ¥4,000,000)
The direct colony thin layr chromatography was firstly applied for rapid identification of phytopathogenic bacteria. The defferentiation of the phytopathogenic bacteria at generic level was available amongClavibacter, Agrobacterium and others. Further, in genus Erwinia, the profiles of E.chrysanthemi and E.carotovora subsp. carotovora were clearly different. These differences at apecies level were also observed in genus Pseudomonas. The results indicated practical usefulness of this easy method for rapid identification of phytopathogenic bacteria. During these experiments, the existence of several groups which had similarities for chromatographic profiles in pseudomonads was found. These groups were Cepacia-type, Syringae-type and Solanacearum-type.
Furthemore, the similarities and diversities of chromatographic profiles among the species of pseudomonads which were proposed to be transferred to a new genus Burkholderia were investigated.
The chromatograms of Pseudomonas cepacia, P.caryophylli, P.glumae, P.gladioli, P.plantarii P.andropogonis, P.solanacearum and P.rubrisubalbicans resembled roughly each other. However, the profiles of three spots which were desibnated as S1, S2 and S3 around Rf0.6 were distinct at species level. The chromatograms of P.andropogonis, P.solaceacesrum and P.rubrisubalbicans were characteristic. Especially, the profile of P.rubrisubalbicans was quite unique in these pseudomonads. So far as the chromatograms for amino-lipids is concerned, some similarities were observed between P.andoropogonis and P.solanacearm. However, P.andropogonis was distinguishable with the existence of single spot which is detectable at Rf0.83 by spraying of the chromium-containing 55% sulfuric acid and heating at 110ﾟC.The usefulness of the direct colony TLC method for rapid identification of phytopathogenic bacteria at species level was repeatedly verified.