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Production of new method on peritoncal-irrigation cytology by use of releasing method of carcinoembryonic antigen by phosphatidylinositol phospholipase C

Research Project

Project/Area Number 06454363
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionGifu University School of Medicine

Principal Investigator

KUNIEDA Katsuyuki  Gifu University School of Medicine Reserch Associate, 医学部, 助手 (60186418)

Co-Investigator(Kenkyū-buntansha) KAWAI Masahiko  Gifu University School of Medicine Reserch Associate, 医学部・附属病院, 助手 (80283307)
SUGIYAMA Yasuyuki  Gifu University School of Medicine Reserch Associate, 医学部, 助手 (90211309)
UMEMOTO Takao  Gifu University School of Medicine Assistant Professor, 医学部・附属病院, 講師 (40223614)
辻 恭嗣  岐阜大学, 医学部・附属病院, 助手 (20262769)
川口 順敬  岐阜大学, 医学部・付属病院, 助手 (50252135)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1996: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsperitoneal dissemination / irrigation cytology / gastric cancer / CEA / phosphatidylinositol phospholipase C / assay system / PIPCL / 診断 / PIPLC
Research Abstract

To get early diagnosis of peritoneal dissemination on gastric cancer, usefulness of a novel method using release of carcinoembryonic antigenA (CEA) from the cancer cells of peritoneal irrigation by Phosphatidylinositol phospholipase C (PI-PLC) was evaluated. Experimental study : 1) The CEA value was increased about 1.7 times by PI-PLC against KATO-III cancer cells. 2) The CEA value increased depend on duration of incubation with PI-PLC and number of target cells. Clinical study : Two kinds of irrigation cytology were performed at laparotomy on 81 gastric cancer patients, that is irrigation cytology (IR) and PI-PLC.1) Positive rate of 12 patients with macroscopic peritoneal dessemination (P) was 91% of IR and 83% of PI-PLC,while it was 2.9% and 7.2% respectively in 69 cases without P.2) CEA sensitibity showed from 1.8 times to 600 times, and characteristic showing positive PI-PLC was the cases suggesting microscopic peritoneal dissemination from clinico-pathological findings. It is sugg … More ested that PI-PLC was a useful method on objective detection for early peritoneal dissemination. By use of PI-PLC,we newly produced a novel kits for peritoneal irrigation cytology. Method : The peritoneal sediment obtained from peritoneal irigation and filtrated by glass microfiber filter were **xed on the plate coated with anti-CEA antibody and added 0.05 U of PIPLC.The positive reaction was given if there was color change between the plate with PIPLC and without it after incubation for 60 minutes and colored by Sandwitch method (PIPLC-kit). The comparison was performed among PIPLC-kit and IR using 53 gastric cancer patients. Results : The 16 patients showing positive reaction by IR were also all positive by PIPLC-kit. While the 37 patients showing negative by IR showed positive by PIPLC-kit. The 42 patients without macroscopic peritoneal metastases showed positive reaction in 5 cases by IR and 10 cases by PIPLC-kit. It is suggested that the PIPLC-kit was useful and simple assay system for the detection of early peritoneal dissemination. Less

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 辻 恭嗣: "胃癌腹膜播種性転移早期診断法としてのphosphatidylinositol phospholipase C を用いたCEA抗原遊出法の有用性について" 日本消化器外科学会誌. 28. 104 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 辻 恭嗣: "phosphatidylinositol phospholipase C を用いたcarcinoembryonic antigen 可溶化の試みと腹腔洗浄細胞診への応用" 日本消化器外科学会誌. 30. 1725-1733 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kyoshi Tsuji, Shigetoyo Saji, Katsuyuki Kunieda, Hiroshi Uenishi, Tuneaki Hatoh, Toshiyuki Hashimoto and Toshiya Hibi: "Uscfulness of Carcinoembryonic Antigen Releasing by Phosphatidylinositol Phospholipase C for Early Diagnosis of Peritoneal Dissemination from Gastric Cancer" The Japanese Journal of Gastroenterological Sergery. Vol.28 No.1. 104 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kyoshi Tsuji, Takashi Suhara, Motohisa Kato, Hiroshi Takao, Yasuyuki Sugiyama, Katsuyuki Kunieda, Takao Umemoto, Kiichi Miya, Daizo Fukada and Shigetoyo Saji: "Significance and usefulness on peritoneal-irrigation cytology by use of releasing method of carcinoembryonic antigen by phosphatidylinositol phospholipase C" The Japanese Journal of Gastroenterological Sergery. Vol.30 No.7. 1725-1733 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 国枝克行: "切除可能胃癌症例予後予測法としての術前・後血清IAP測定の意義について" BIOTHERAPY. 6・5. 663-665 (1992)

    • Related Report
      1996 Annual Research Report
  • [Publications] 国枝克行: "胃癌患者における血清CA72-4の測定意義について-血清CEA,CA19-9との比較から-" 癌の臨床. 38・10. 1077-1082 (1992)

    • Related Report
      1996 Annual Research Report
  • [Publications] 国枝克行: "胃癌症例における術中腹腔内洗浄細胞診の評価-とくに漿膜面浸潤面積および漿膜面肉眼分類との関係-" 日本臨床外科医学会誌. 54・5. 1167-1172 (1993)

    • Related Report
      1996 Annual Research Report
  • [Publications] 杉山保幸: "再発・転移形式別にみた胃癌治療の工夫" 日本消化器外科学会誌. 27・10. 2293-2297 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] 辻 恭嗣: "胃癌腹膜播種性転移早期診断法としてのPhospatidylinositol-phospholipase Cを用いたCEA抗原遊出法の有用性について" 日本消化器外科学会誌. 28・1. 104 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 国枝克行: "胃癌術前血清CEA値および組織CEA局在様式と予後との関連について" 日本消化器外科学会誌. 29・3. 677-683 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 国枝克行: "切除可能胃癌症例予後予測法としての術前・後血清IAP測定の意義について" BIOTHERAPY. 6. 663-665 (1992)

    • Related Report
      1995 Annual Research Report
  • [Publications] 国枝克行: "胃癌患者における血清CA72-4の測定意義について-血清CEA, CA19-9との比較から-" 癌の臨床. 38. 1077-1082 (1992)

    • Related Report
      1995 Annual Research Report
  • [Publications] 国枝克行: "胃癌症例における術中腹腔内洗浄細胞診の評価-とくに漿膜面浸潤面績および漿膜面肉眼分類との関係-" 日本臨床外科医学会誌. 54. 1167-1172 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] 杉山保幸: "再発・転移形式別にみた胃癌治療の工夫" 日本消化器外科学会誌. 27. 2293-2297 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 辻 恭嗣: "胃癌腹膜播種性転移早期診断法としてのphospatidylinositol-phospholipasecを用いたCEA抗原遊出法の有用性について" 日本消化器外科学会誌. 28. 104 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 国枝克行: "胃癌術前血清CEA値および組織CEA局在様式と予後との関連について" 日本消化器外科学会誌. 29. 677-683 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 國枝克行: "切除可能胃癌症例予後予測法としての術前・後血清IAP測定の意義について" BIOTHERAPY. 6. 663-665 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] 國枝克行: "胃癌患者における血清CA72-4の測定意義について-血清CEA,CA19-9との比較から-" 癌の臨床. 38. 1077-1082 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] 國枝克行: "胃癌症例における術中腹腔内洗浄細胞診の評価-とくに漿膜面浸潤面積および漿膜面肉眼分類との関係-" 日本臨床外科医学会誌. 54. 1167-1172 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 杉山保幸: "再発・転移形式別にみた胃癌治療の工夫" 日本消化器外科学会誌. 27. 2293-2297 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 辻 恭嗣: "胃癌腹膜播種性転移早期診断法としてのPhospatidylinositol phospholipasecを用いたCEA抗原遊出法の有用性について" 日本消化器外科学会誌. 28. 104 (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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