|Budget Amount *help
¥7,600,000 (Direct Cost : ¥7,600,000)
Fiscal Year 1995 : ¥800,000 (Direct Cost : ¥800,000)
Fiscal Year 1994 : ¥6,800,000 (Direct Cost : ¥6,800,000)
The right sciatic nerve of inbred Lewis rats was cut at the central region and the distal tip was transected at the muscle entry site.
Allograft was made of the anterior horn of spinal cord of PVG rats in the TA muscle of the rats treated as described above, following allograft, administration of immunosuppresant (FK506) was made daily.
At 4,8,24 weeks, measurement of the wet weight of the TA muscle, twitch tension, and tetanic tension of each group was made, followed by histological examination of the TA muscle. In the graft group, the value of the wet weight, twitch tension, tetanic tension tended to be higher than those in the control group, but the difference was not statistically significant. Furthermore, survival of anterior horn cells of grafted spinal cord grafted in the TA muscle could not also be confirmed histologically. Therefore, in order to examine the condition of the grafted spinal cord cells during the early postoperative period, that is, from 1 day to 1 week postoperatively, histological study was made during the early postoperative period. It was confirmed by this study that necrosis developed in the anterior horn cells grafted in the muscle from the early postoperative period with no survival of these cells. The histological study suggested that allograft of anterior horn cells was not rejected by immuno-response and that the environment surrounding the cells was inadequate for cell survival. AS it was considered that the primary problem point involved was how to achieve survival of the grafted anterior horn cells, study now in progress by grafting fetal spinal cord outstanding nerve regeneration capacity.