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ROLE OF PINEAL HORMONE IN THE REGULATORY MECHANISM FOR EGG-LAYING OF POULTRY

Research Project

Project/Area Number 06660362
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Applied animal science
Research InstitutionTOKYO UNIVERSITY OF AGRICULTURE

Principal Investigator

TANAKA Katsuhide  TOKYO UNIVERSITY OF AGRICULTURE, Faculty of Agriculture, PROF., 農学部, 教授 (20021678)

Co-Investigator(Kenkyū-buntansha) KAWASHIMA Mitsuo  GIFU UNIV., ANIM.PROD.SCI., PROF., 農学部, 教授 (10177686)
KUWAYAMA Takehito  TOKYO UNIVERSITY OF AGRICULTURE, Faculty of Agriculture, LECTURER, 農学部, 講師 (40215124)
MONJI Yasunori  TOKYO UNIVERSITY OF AGRICULTURE, Faculty of Agriculture, ASSOC.PROF., 農学部, 助教授 (50096682)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordschicken / pineal hormone / melatonin receptor / rediologand assay / ovulatory cycle / hypothalamus / anterior pituitary / granulosa cell / 家禽 / 産卵機能 / ラジオリガンドアツセイ / ラジオリガンドアッセイ
Research Abstract

Plasma membrane fractions of tissues of the hypothalamic preoptic area and median eminence area, the anterior lobe of pituitary, and the ovarian follicular granulosa and threca cells were analyzed by radioligand assays for binding properties to tritium labeled metatonin, the pineal hormone. The membrane fractions of all of the tissues except for those of the ovarian follicular theca cells revealed the binding specificity for melatonin among melatonin-related substances and the saturable binding to ^3H-melatonin. By Scatchard analyzes of the specific binding to ^3H-melatonin, the binding component was found to have a single class of binding sites, a high affinity and a limited capacity, indicating that the binding component can be regarded as being a receptor for melatonin. The binding affinity and capacity in the hypothalamic preptic area and median eminence area changed during an ovulatory cycle in laying hens and during a 24h period in nonlaying hens, but the pattern of the change was not different between the laying and nonlaying hens but different between the two hypothalamic areas. The specific binding to ^3H-melatonin in the anterior lobe of pituitary changed during the ovulatory cycle in laying hens, but did not change during a 24-h period in nonlaying hens, suggesting that the binding of melatonin receptor in the pituitary may be related to the egg-laying. The results of the present study suggest a direct action of melatonin on the hypothalamus, the pituitary, and the ovarian follicular granulose cells in the hen.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Murayama,T.,Kuwayama,T.,Monji,Y.and Tanaka,K.: "Detection of specific binding component to pineal hormone,melatonin,in hypothalamo-pituitary-gonadal axis of the chicken" Proc. 7th AAAP Animal Science Congress. 2. 421-422 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] MURAYAMA, T., KUWAYAMA, T., MONJI, Y., AND TANAKA, K.: "Detection of specific binding component to pineal hormone, melatonin, in hypothalamo-pituitary-gonadal axis of the chicken" Proc. 7th AAAP Animal Science Conqress. 2. 421-422 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Murayama, T., Kuwayama, T., Monji, Y., and Tanaka, K.: "Detection of specific binding co mponent to pineal hormone, vnelatonin, in hypothalamo-pituirary-qonadal axis of the chicken" Proc. 7th AAAP Animal Science Congress. 2. 421-422 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 村山透,桑山岳人,門司恭典,田中克英,川島光夫: "Detection of specific binding component to pineal hormone,melatonin,in hypothalamo-pituitary-gonadal axis of the chicken" Proc.7th AAAP Animal Science Congress. 2. 421-422 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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