• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of platelet-derived growth factor in cerebral ischemia, by gene cloning and by in situ hybridization.

Research Project

Project/Area Number 06670223
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionShiga University of Medical Science

Principal Investigator

SASAHARA Masakiyo  Shiga Univ.of Med.Sci., Faculty of Medicine Assistant, 医学部, 助手 (20154015)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordscloning / in situ hybridization / neuron / platelet-derived growth factor / gene / transplantation / gliosis / ischemia / 脳 / マクロファージ / 外傷
Research Abstract

Previously, we found platelet-derived growth factor-B chain (PDGF-B) and its receptor expression in monkey brain. Here, in this project, we demonstrated, 1) PDGF-B expression in normal rat brain : In normal and mature rat brain, we have determined the localization of PDGF-B gene in neurons by in situ hybridization histochemistry. These transcripts were almost 3.5 and 2.6 kb, as shown by northern blot. The protein expression was confirmed by the detection of PDGF-B-related mitogenic activity in the brain. 2) Regulation of PDGF-B expression related to cerebral insults and transplantation : Rapid and transient increase of PDGF-B expression was induced in the neurons in the vicinity of the lesions, such as focal cerebral ischemia and hypoxic/ischemic injury. In the transplantation study of rat olfactory bulb, PDGF-B expression was observed in a close proximity to the radiation of individual axon from olfactory nerve bundles to surrounding neuropil. These findings obtained in cerebral injur … More ies and transplantation model suggest that PDGF-B should play a role in the protection of neurons at risk, and in the remodeling of neural fiber network, together with previously demonstrated its neurotrophic activity to cultured neuronal cells. Following focal cerebral ischemia, transient PDGF-B upregulation was followed by increase of PDGF-b receptor expression in cells such as neurons, reactive glial cells and macrophages. After cerebral stab wound, the expression of PDGF-B in macrophages correlated with reactive astrogliosis.These results obtained in focal ischemic injury and stab wound further suggest the role of PDGF-B in the healing process of the injured brain. 3) Cloning of the PDGF-B gene from brain : Immediately after focal cerebral ischemia, rapid induction of PDGF-B gene of short form was observed. We successfully cloned its gene from brain by use of anchored PCR cloning. Long untranslated 5'-end sequence of PDGF-B gene has been shown to inhibit its protein translation. In the present study, short transcripts which increased after cerebral injury was determined as truncated form, lacking their 5'-end untranslated sequence. Novel regulatory mechanism of protein expression was demonstrated, that is, induced truncated gene expression, lacking translation inhibitory sequence in 5'-end untranslated region, supply template for PDGF-B protein translation. 4) In summary, we have determined PDGF-B gene expression in neurons and its expression related to insults and transplantation. Further, gene cloning from brain demonstrated novel regulatory mechanism for the protein expression. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (50 results)

All Other

All Publications (50 results)

  • [Publications] Sasahara Masakiyo: "Inhibition of hyperecholesterolemia-induced atherosclecrosis in nonhuman primate by producol : I. Is the extent of atherosclerosis related to resistance of LDL to oxidation?" J. Clin. Invest.94. 155-164 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Alan Chait: "Oxidative modification of lipoproteins and their treatmentin primates" Atherosclerosis.109. 84-85 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sasahara Masakiyo: "Analysis of secretion and expression of platelet-derived growth factor expression in cultured endothelial cells from stroke-prone spontaneously hypertensive rat" J. Cell Biochem., suppl.18A. 272 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Chang Y.Mary: "PCNA positive cells are decreased in lesions of probucol-treated hypercholesterolemic monkeys" J. Cell Biochem. suppl.18A. 265 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hayase Yoneko: "Analysis of platelet-derived growth factor expression in cultured aortic endothelial cells from stroke-prone spontaneously hypertensive rats" Atherosclerosis. 109. 184 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hayase Yoneko: "Enhanced expression of PDGF-B chain mRNA in the cultured aortic endothelial cells from stroke-prone SHR." Jpn Heart J. 35. 519 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sasahara Masakiyo: "Platelet-derived growth factor b-chain comprises the major part of enhanced released mitogen from aortic endothelial cells of stroke-prone spontaneously hypertensive rats" Clin. Exp. Pharmacol. Physiol. suppl. 1. S123-125 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hazama Fumitada: "Pathogenesis of lacuna-like cyst formation and diffuse degeneration ot the white matter in the brain of stroke-prone spontaneously hypertensive rats" Clin Exp Pharm Pysiol.suppl. 1. S260-261 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sasahara Masakiyo: "Expression of Platelet-Derived Growth Factor B-Chain in the Mature Rat Brain and Pituitary Gland" Mol. Brain Res.32. 63-74 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Uchida Kazunori: "Expression of platelet-derived growth factor B-chain in neointimal smooth muscle cells of balloon injured rabbit femoral arteries" Atherosclerosis. (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Iihara Kouji.: "Induction of platelet-derived growth factor β-receptor in focal ischemia of rat brain" J. Cereb. Blood Flow Metab.(1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Chang Y. Mary: "Inhibition of hypercholesterolemia-induced atherosclerosis in the nonhuman primate by probucol : II. cellular composition and proliferation" Arterioscler,Thromb. Vasc. Biol.15. 1631-1640 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sasahara Masakiyo: "Regulation of gene and protein expression of platelete-derived growth factors and their receptors in developing and injured rat brains." 4th IBRO World Congress of Neuroscience.146 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 内田和則: "家兎大動脈バル-ニングモデルにおけるPDGF-B鎖mRNAおよび蛋白発現と細胞増殖の関連" 日本病理学会誌. 第84巻. 148 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 大野正樹: "新生仔ラット低酵素性虚血性障害脳におけるPDGF-B鎖の発現" 日本病理学会誌. 第84巻. 223 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 笹原正清: "局所脳虚血における血小板由来増殖因子受容体の発現" 日本病理学会誌. 第84巻. 269 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sasahara Masakiyo: "Enhanced expression of platelet-derived growth factor (PDGF) B-chain and its receptor in focal ischemiain rat brain" 日本神経病理学会. 第15巻. 79 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 1) Sasahara Masakiyo et al.: "Inhibition of hyper-chlolesterolemia-induced atherosclerosis in the nonhuman primate by probucol : I.Is the extent of atherosclerosis related to resistance of LDL to oxidation?" J.Clin.Invest.94. 155-164 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 2) Takayama Syoukei et al.: "Platelet-derived growth factor B-chain-like immunoreactivity in injured rat brain." Brain Res.653. 131-140 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 3) Iihara Kouji et al.: "Ischemia induces the expression of PDGF-B chain in neurons and brain macrophages in vivo" J.Cereb.Blood Flow Metab.14. 818-824 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 4) Alan Chait et al.: "Oxidative modification of lipoproteins and their treatment in primates" Atherosclerosis. 109. 84-85 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 5) Sasahara Masakiyo, et al.: "Analysis of secretion and expression of platelet-derived growth factor in cultured endothelial cells from stroke-prone spontaneously hypertensive rat." J.Cell Biochem., suppl.18A. 272 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 6) Chang Y.Mary et al.: "PCNA positive cells are decreased in lesions of probucol-treated hypercholesterolemic monkeys" J.Cell Biochem.suppl.18A. 265 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 7) Sasahara Masakiyo: "Platelet-derived growth factor b-chain comprises the major part of enhanced released mitogen from aortic endothelial cells of stroke-prone spontaneously hypertensive rats" Clin.Exp.Pharmacol.Physiol.suppl.1. 123-125 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 8) Hazama Fumitada et al.: "Pathogenesis of lacuna-like cyst formation and diffuse degeneration of the white matter in the brain of stroke-prone spontaneously hypertensive rats" Clin Exp Pharm Physiol.suppl.1. 260-261 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 9) Sasahara Masakiyo et al.: "Expression of Platelet-Derived Growth Factor B-Chain in the Mature Rat Brain and Pituitary Gland" Mol.Brain Res.32. 63-74 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 10) Uchida Kazunori et al.: "Expression of platelet-derived growth factor B-chain in neointimal smooth muscle cells of balloon injured rabbit femoral arteries" Atherosclerosis. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 11) Iihara Kouji.et al.: "Induction of pltelet-derived growth factor beta-receptor in focal ischemia of rat brain" J.Cereb.Blood Flow Metab.(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 12) Chang Y.Mary et al.: "Inhibition of hypercholesterolemia-induced atherosclerosis in the nonhuman primate by probucol : II.cellular composition and proliferation" Arterioscler, Thromb.Vasc.Biol.15. 1631-1640 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 13) Sasahara Masakiyo et al.: "Regulation of gene and protein expression of platelet-derived growth factors and their receptors in developing and injured rat brains." 4th IBRO World Congress of Neuroscience, Oxford.New York. 146 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sasahara Masakiyo: "Inhibition of hypercholesterolemia-induced atherosclerosis in the nonhuman primate by probucol:I.Is the extent of atherosclerosis related to resistance of LDL to oxidation?" J.Clin.Invest.94. 155-164 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Alan Chait: "Oxidative modification of lipoproteins and their treatment in primates" Atherosclerosis.109. 84-85 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sasahara Masakiyo,: "Analysis of secretion and expression of platelet-derived growth factor in cultured endothelial cells from stroke-prone spontaneously hypertensive rat," J.Cell Biochem.,suppl.18A. 272 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Chang Y.Mary.,: "PCNA positive cells are decreased in lesions of probucol-treated hypercholesterolemic monkeys," J.Cell Biochem.suppl.18A. 265 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hayase Yoneko: "Analysis of platelet-derived growth factor expression in cultured aortic endothelial cells from stroke-prone spontaneously hypertensive rats" Atherosclerosis,. 109. 184 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hayase Yoneko: "Enhanced expression of PDGF-B chain mRNA in the cultured aortic endothelial cells from stroke-prone SHR." Jpn Heart J. 35. 519 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] SASAHARA Masakiyo: "Expression of platelet-derived growth factor(PDGF-B-chain)in developing and mature rat brain," The eighth international conference of the international society of differentiation(ISD). 124- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] TAKAYAMA Syoukei: "Platelet-derived growth factor B-chain-like immunoreactivity in injured rat brain." Brain Res.653. 131-140 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] IIHARA Koji: "Ischemia induces the expression of PDGF-B chain in neurons and brain macrophages in vivo" J.Cereb.Blood Flow Metab.14. 818-824 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kott,J.N.: "Olfactory ensheathing glia and platelet-derived growth factor B-chain reactivity in the transplanted rat olfactory bulb," Int.J.Devl.Neuroscience. 12. 315-323 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] TAKAYAMA Syoukei: "Platelet-derived growth factor B-chain like immunoreactivity in injured rat brain," Brain Pathology. 4. 490- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] IIHARA Koji: "Coordinate expression of platelet-derived growth factor-B chain and β-receptor in focal ischemia of rat brain," Stroke. 25. 267- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] IIHARA Koji: "Induction of Platelet-derived growth factor b-receptor in focal ischemia of rat brain" Brain Pathology. 4. 509- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] SASAHARA Masakiyo: "Expression of platelet-derived growth factor B-chain in the mature rat brain and pituitary gland" Mol.Brain Res. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 笹原正清: "ラット脳における血小板由来増殖因子(PDGF)-B鎖mRNA発現のin situハイブリダイゼーションによる解析" 日本病理学会会誌. 83. 208- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 笹原正清: "ラット脳損傷における血小板由来増殖因子(PDGF)の発現、" 日本病理学会会誌. 83. 272- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 笹原正清: "局所脳虚血における血小板由来増殖因子(PDFG)-B鎖" 日本病理学会会誌. 83. 272- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 大野雅樹: "ラット新生仔低酸素性虚血性脳障害における血小板由来増殖因子(PDGF)の発現、" 日本神経病理学会会誌. 35. 177- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 笹原正清: "成熟ラット脳における血小板由来増殖因子B-鎖発現の解析" 日本神経病理学会会誌. 35. 201- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 高山昌奎: "砂ネズミ一過性前脳虚血モデルにおける血小板由来増殖因子B-鎖の発現" Neurologia medico-chirurgica. 53. 356- (1994)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi