|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1995 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 1994 : ¥1,600,000 (Direct Cost : ¥1,600,000)
As for invarsion and metastasis to local site of cancer cells with cancer patients, the inhibition becomes important theme by bringing major influence for prognosis. Recently, the growth factor producced tumor is reported related to the organs selectivity of cancer metastasis by a mouse tumor. Previously, we found a stimulant substance for immuno competent cells with production from human lung cancer cells, the same factor with related to metastasis of lung cancer, we thought that did participate in cell movement to metastasis process of lung cancer and the control of autocrine/paracrine growth factor with tumor local site, then we studied the role in metastasis as an indicator with gene of produced growth factor by molecular biolog. The seventeen cells lines of malignant tumor including 13 lung cancer cells was using.
TGF-beta mRNA was expressed in seventeen cells lines of malignant tumor. The mRNA of cytokine IL-8, IL-1alpha and IL-6 was expressed 80%, 65% and 35%, respectively. Further we analyzed for related to tumor metastasis in those cells. E-cadoherin, CD44 and c-met mRNA was expressed in 13 lines, 8 lines and 12 lines, respectively. Inversive activity by matrigel was shown in 10 cell lines. And the inversive activity of those cells related to the adhesion for matrigel and activation of metalloprotinases.
Those results suggest the numerous cytokines playd an effective role for tumor progression through the cell interactions in microenvironment.