ROLE OF NALT IN MUCOSAL IMMUNITY OF UPPER
Project/Area Number |
06671729
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Research Category |
Grant-in-Aid for General Scientific Research (C)
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Allocation Type | Single-year Grants |
Research Field |
Otorhinolaryngology
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Research Institution | SAPPORO MEDICAL UNIVERSITY |
Principal Investigator |
KOHJI Asakura SAPPORO MEDICAL UNIV,OTOLARYNGOL,ASSOCIATE PROFESSOR, 医学部, 助教授 (50136925)
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Co-Investigator(Kenkyū-buntansha) |
OGASAWARA Hideki SAPPORO MEDICAL UNIV,OTOLARYNGOL,INSTRUCTOR, 医学部, 助手 (00271148)
NARITA Shinichiro SAPPORO MEDICAL UNIV,OTOLARYNGOL,INSTRUCTOR, 医学部, 助手 (30264526)
OGURO Shinji SAPPORO MEDICAL UNIV,OTOLARYNGOL,INSTRUCTOR, 医学部, 助手 (40253992)
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Project Period (FY) |
1994 – 1995
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Project Status |
Completed (Fiscal Year 1995)
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Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
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Keywords | NALT / Rats / Immune response / 上気道粘膜免疫 / 抗原特異的免疫グロブリン / アデノイド / 口蓋扁桃 / 抗体産生細胞 |
Research Abstract |
Nasopharyngeal associated lymphoid tissue (NALT) in rats appears to be equivalent to the human adenoid and tonsil, and to have a role in mucosal immune responses of the upper respiratory tracts. We examined the antigen-specific immunoglobulin (lg) contents in nasopharyngeal lavage fluid and antigen specific lg production by NALT lymphocytes in rats immunized by various routes. A significant increase of antigen-specific lgA level, as well as in the lgG level, was noted in the nasopharyngeal lavage fluid of immunized animals. OA-specific lgG spot forming cells (SFC) in NALT lymphocytes was higher than those in spleen lymphocytes in locally immunized rats, whereas inverse relationships were noted in generally immunized rats. These OA-specific lgG SFC were remarkably increased in NALT,but not so increased in the spleen following nasal booster challenge. The numbers of OA-specific lgA SFC in freshly obtained lymphocytes were very low in both NALT and spleen even after the topical antigen challenge. However, OA-specific lgA SFC were revealed to be remarkably increased after in vitro stimulation with antigen or lipopolysaccaride (LPS). Further increases of antigen-specific lgA as well as lgG SFC in NALT lymphocytes occurred in the presence of TFG-beta and IL-5.
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Report
(3 results)
Research Products
(4 results)