| Project/Area Number |
06671797
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
小児外科
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| Research Institution | Nihon University |
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Principal Investigator |
IWATA Mitsumasa Nihon University, School of Medicine, Assistant Professor, 医学部, 講師 (60160123)
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| Co-Investigator(Kenkyū-buntansha) |
MATUDA Takesi Nihon University, School of Medicine, Assistant, 医学部, 助手 (90267063)
KOSHINAGA Tsugumichi Nihon University, School of Medicine, Assistant, 医学部, 助手 (70205376)
OKABE Ikuo Nihon University, School of Medicine, Professor, 医学部, 教授 (20059017)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Apoptosis / Neuroblastoma / Spontaneous Reduction / Molecula Biology / apoptosis |
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| Research Abstract |
The purpose of this study is to make the relationship clear between the spontaneous tumor reduction and apoptosis in neuroblastoma using molecular biologocal technics. Only 20% cases were demonstrated apoptotic change by usual electrophoresis stained with ethidium bromide, but 100% by isotope labelling method which used to elevate the sensitivity (Frank method). The degree of apoptosis showed no significant diference between the tumor found by mass screening and that of advanced one found by clinically
Flow cytometric study were also done to measure the proliferating rate of the tumor (Proliferating Index : PI sacore). PI score was lower in the tumor found by mass screening than the tumor found clinically.
We speculate that spontaneous tumor reduction may occure if the degree of apoptotic change overcome that of proliferation.
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