| Project/Area Number |
06671810
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
YAMAAI Yuichiro Okayama University, Dental School, Research assistant, 歯学部, 助手 (00158057)
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| Co-Investigator(Kenkyū-buntansha) |
ICHIKAWA Hiroyuki Okayama University, Dental School, Professor assistant, 歯学部, 助教授 (20193435)
SUGIMOTO Tomosada Okayama University, Dental School, Professor, 歯学部, 教授 (50135729)
KOYAMA Eiki Okayama University, Dental School, Research assistant, 歯学部, 助手 (30186829)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Mouse / Tooth germ / ZPA / Grafting / Chick limb bud / In situ hybridization / モルフォゲン / 移植 / 重復肢誘導 / マウス / 歯胚 / 間葉 |
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| Research Abstract |
Tooth development involves reciprocal epithelio-mesenchymal interactions and complex morphogenetic events. Because these processes bear similarities to those occurring in the developing limb, we asked whether signaling mechanisms found in the limb also occur in the tooth. We grafted mouse embryonic tooth germs to the anterior margin of the host chick embryonic wing buds and determined whether the tooth tissues had polarizing activity. Indeed the grafted tooth germs induced the formation of the ectopic digits as a miller image as well as the surface structures. These activity of the molar tooth germ increased from bud to cap stage and reached at the maximum at late bell stage. With further development, the polarizing activity began to decrease, however, once it became the highest level at neonates. And then the activity decreased to undetectable level at 3-week old.
In situ hybridization demonstrated that the tooth germ grafts induced expression of chick HoxD cluster genes prior to induction of ectopic digits. We concluded that the factors produced by the tooth germs were similar to those in the zone of polarizing activity in the limb development and might regulate morphogenesis and differentiation in the molar tooth development.
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