|Budget Amount *help
¥2,000,000 (Direct Cost : ¥2,000,000)
Fiscal Year 1996 : ¥600,000 (Direct Cost : ¥600,000)
Fiscal Year 1995 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 1994 : ¥700,000 (Direct Cost : ¥700,000)
1) Effect of EGF in paracrine manner
In order to clear the effects of Epidermal Growth Factor (EGF) on the invasion abilities of human oral squamous cell carcinoma, five cell lines were studied. EGF enhanced the random motility of all cell lines in a dose-dependent fashion and exposure to EGF let to an increased production of urokinase type plasminogen activator and matrix metalloproteniase-9 by the same cells. These results strongly suggest the EGF may promote the invasion and metastasis of human oral squamous cell carcinomas. (Tumor Biology, 17 : 168-175,1996.)
2) EFFECT OF EGF IN AUTOCRINE MANNER
Cell lines, which produced EGF,was found out in these cell lines. In these cells, EGF produced by tumor cells can stimulate the random motility itself through the EGF receptor. (Oncologia 27 : 455-457,1994)
3) ESTABLISHMENT OF EGF SENSITIVE CLONE
To reveal the signal pathway, we established s-1 clone cells, which was most sensitive clone against the EGF stimulation and also we established i-1 clone cells, which was most insensitive clone. Comparison of these clone cells may lead the mechanisms of signal transduction of EGF-induced motility in near future.
4) ANALYSIS OF SIGNAL TRANSDUCTION OF EGF-INDUCED MOTILITY.
When EGF bind to the EGF receptor, tyrosine phosphorelation is firstly occurred and subsequently PKC is activated through the activation of PLCr arising from erbB homodimer and/or activation of PI3-kinase arising from erbB3 heterodimer. (Proceeding of JJCR,54th Annual Meeting, P458)