|Budget Amount *help
¥2,200,000 (Direct Cost : ¥2,200,000)
Fiscal Year 1995 : ¥400,000 (Direct Cost : ¥400,000)
Fiscal Year 1994 : ¥1,800,000 (Direct Cost : ¥1,800,000)
Vascular endothelial cells contact with blood in vivo form no thrombi in the normal state. Artificial polymer materials where human vascular endothelial cells (HUVEC) are seeded may be expected to lead to antithrombogenic surfaces. Such hybrid materials will be useful as artificial blood vessels for a long-term implantation. Vascular endothelial cells, however, stimulated with cytokines, are known to change to the procoagulant state.
In this study, the compatibility of HUVEC with artificial materials was investigated on fibrinolysis-related proteins, i.e., tissue-type plasminogen activator (tPA) and plasminogen inhibitor type 1 (PAI-1). The following results were obtained ;
1) HUVEC were observed to be released relatively high amount of tPA in the initial culture period for four days.
2) The activity of tPA,measured with a fluorogenic synthetic substrate, was scarcely detected in the culture medium of HUVEC,while the activity of tPA was detected in the HUVEC lysate component in which PAI-1 was inactivated. Probably, tPA,released from HUVEC,should form complex with PAI-1 in the culture medium.
3) The ratio of amount of tPA against that of PAI-1, released in the culture medium, was dependent on extracellular matrices (ECM), i.e., ECM proteins and artificial materials used.