|Budget Amount *help
¥2,000,000 (Direct Cost : ¥2,000,000)
Fiscal Year 1995 : ¥1,000,000 (Direct Cost : ¥1,000,000)
Fiscal Year 1994 : ¥1,000,000 (Direct Cost : ¥1,000,000)
To clarify the physiological functions of the Rh-blood group system, we investigated the contribution of D-antigen to various transport systems using red-blood-cell-ghosts (RBC-ghosts) of various Rh-phenotypes. Namely, glucose, p-aminohippuric acid, tetraethyl-ammmonium, and glycylsarcosine were used as the model drugs for the transport system of glucose, organic anion, organic cation, and dipeptide, respectively. Drug uptake experiments were done by the rapid membrane filtration method using 3H- or 14C- labeled drugs.
The uptake of p-aminohippuric acid was increased with an outside-to-inside proton gradient, and that of the glycylsarcosine was increased with an inside-to-outside proton gradient. However, the uptake of these drugs was not affected by the Na+-K+gradient, drug concentrations, or inhibitors. Anti-D antibodies did not affect the uptake of these drugs with RBC-ghosts. No differences in the uptake properties were observed with the Rh-phenotype. These findings showed that the active transport systems and D-antigen did not contribute to these processes. Thus, the effect of the proton gradient observed for p-aminohippuric acid and glycylsarcosine may be due to the pH-partition theory.