Grant-in-Aid for international Scientific Research
|Allocation Type||Single-year Grants|
|Research Institution||Nagoya University School of medicine|
KAWAMOTO Fumihiko Dept.of Med.Zool., School of Medecine Nagoya Univ.Lecturer, 医学部, 講師 (40115556)
TANTULAR Indah Dept.of Parasitol., Fac.of Med., Airlagga Univ., Indonesia, 医学部, 講師
WIRJATMADI Bamban Dept.of Parasitol., Fac.of Med., Airlangga Univ., Indonesia, 医学部, 講師
DACHLAN Yoes.P Dept.of Parasitol., Eac.of Med., Airlangga Univ., Indonesia, 医学部, 助教授
WONGSRICHANA チャンスタ アメリカ陸軍医科学研究所, 研究員
YAMAMOTO Toshimichi Dept.of Forns.Sc., Sch.Med.Nagoya Univ., 医学部, 助手 (50260592)
近藤 高明 名古屋大学, 医学部, 助手 (00195900)
松岡 裕之 自治医科大, 講師 (10173816)
KIMURA Masatsugu Lab.of biophysics, Med.Sch.Osaka City Univ., 医学部, 助手 (60195378)
綿矢 有佑 岡山大学, 薬学部, 教授 (90127598)
WONGSRICHANALAI Chansuda Dept.of Immunol., Armed Forc.Res.Inst.Med.Sc., Bangkok
CHANSUDA Won アメリカ陸軍医科学研究所, 研究員
WIRJATMADI バンバン アイルランガ大学, 医学部, 講師
|Project Period (FY)
1995 – 1996
Completed(Fiscal Year 1996)
|Budget Amount *help
¥7,400,000 (Direct Cost : ¥7,400,000)
Fiscal Year 1996 : ¥3,800,000 (Direct Cost : ¥3,800,000)
Fiscal Year 1995 : ¥3,600,000 (Direct Cost : ¥3,600,000)
|Keywords||Malaria / Molecular diagnosis / Acridine orange staining / New variant / Thailand / Indonesia / Myanmar / China / 分子疫学 / 熱帯熱マラリア / 四日熱マラリア / リボゾームRNA遺伝子|
Field survey of malaria was conducted in Thailand, Indonesia, Myanmar and China using an acridine orange (AO) staining method. Diagnostic results were compared with those by microtiter plate hybridization (MPH), and a nested PCR methods.
1. In Thailand, a total of 548 samples were obtained, and many P.malariae and P.ovale infections diagnosed by the AO method were confirmed by the MPH and/or nested PCR methods. Their prevalence reached about 20% and 2.2%, respectively. In addition, three samples of P.ovale infections were AO-positive but MPH-negative. Analysis of the target sequences revealed that these isolates were the same mutated type (two nucleotide deletion and one nucleotide substitution) with that found in Vietnam. On the other hand, P.falciparum isolates were found to possess a point mutation at the target region in the 18S rRNA gene.
2. In China, many P.malariae infections were also detected from the Sichuan Province by the three methods. By the nested PCR method, a long deletion from amplified PCR product was found at the probe region, indicating that this may be a new mutated type of P.malariae. Later, the same mutated form was detected from Thailand, Indonesia, and Myanmar, indicating that it is widely distributing in SEA.
3. In Myanmar, many P.malariae and P.ovale infections were also detected. The mutated types of both of P.malariae and P.ovale which had been found in Vietnam was also detected. In contrast, in Indonesia only six samples of P.malariae and only two of P.ovale were found. One sample of P.malariae was the same new type, but others were normal.