Grant-in-Aid for International Scientific Research.
|Section||Joint Research .|
|Research Institution||TOKYO INSTITUTE OF TECHNOLOGY|
HOSHI Motonori Dept.of Life Sci., Tokyo Institute of Technology Professor, 生命理工学部, 教授 (20012411)
PEIJUN Zhang 中国科学院, 海洋研究所, 教授
西駕 秀俊 東京都立大学, 理学部, 助教授 (60131918)
大日方 昂 千葉大学, 理学部, 教授 (40012413)
森沢 正昭 東京大学, 理学部, 教授 (40013594)
千葉 和義 東京工業大学, 生命理工学部, 助手 (70222130)
松本 緑 東京工業大学, 生命理工学部, 助手 (00211574)
西田 宏記 東京工業大学, 生命理工学部, 助教授 (60192689)
山上 健次郎 上智大学, 理工学部, 教授 (50011474)
ZHANG Peijun Experimental Marine Biology laboratory Institute of Oceanology, Academia Sinica, 海洋研究所, 教授
OBINATA Takashi Tokyo Metropolitan University Fac.of Science, Chiba Univ.
SAIGA Hidetoshi Dept.of Biology, Faculty of science
CHIBA Kazuyoshi Dept.of Life Sci., Tokyo Inst.of Tech.
MORISAWA Masaaki Misaki Marine Biological Station, Univ.of Tokyo
NISHIDA Hiroki Dept.of Life Sci., Tokyo Inst.of Tech.
MATSUMOTO Midori Dept.of Life Sci., Tokyo Inst.of Tech.
|Project Fiscal Year
1995 – 1996
Completed(Fiscal Year 1996)
|Budget Amount *help
¥4,200,000 (Direct Cost : ¥4,200,000)
Fiscal Year 1996 : ¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1995 : ¥2,100,000 (Direct Cost : ¥2,100,000)
|Keywords||oogeneesis / myogenesis / homeotic gene / sperm motility / hatching enzyme / genetic polymorphism / 卵成熟 / 筋形成 / ホメオボックス遺伝子 / 精子運動 / 孵化酵素 / 遺伝的多型 / ナメクジウオ / 原索動物 / 筋肉分化 / トロポニンT / 精子の運動能獲得 / 実験動物化|
In the collaboration research group, we are mainly in charge of collecting mature amphioxus, keeping and feeding them in the lab and supplying the materials such as sperm, eggs, embryos from various stages and tissues etc.for deferent purposes of research. During the amphioxus spawning seasons in 1995 and 1996, we collected 5 kg of the adult amphioxus each season and supplied enough experimental materials for observation and analysis almost everyday.
Embryogenesis of amphioxus was observed and recorded. The eggs do not show conspicuous ooplasmic segregation. The embryos showed regulative development when one of two blastomers were isolated at the 2-cell stage. We had some trials to clone the amphioxus gene homolog of the homeotic gene LIM,which is related to eary embryogenesis, troponin T which is muscle protein and astacin-like protease which is related to haching embryo. Isolation of LIM homeobox gene is now under way by determining nucleotide sequence of the PCR products, followed by screening of the cDNA library, and troponin T is now screening of the cDNA library, but we have no clone of the hatching enzyme. It is hoped that we succeed in the cloning after reformation of the possible problems in the next time. The effects of ion channel antagonists on the motility of amphioxus sperm and motility of the sperm of which the plasma membrane was removed using detergent were investigated. Obtained resutls suggest that fertilization, development and sperm motility are similar to that of marine invertebrates. Genetical variety of rRNA gene (rDNA) repeated units was studied in amphioxus. We detected the variety of the length of the internal spacer region between 18S rDNA and 5.8S rDNA.