Grant-in-Aid for international Scientific Research
|Allocation Type||Single-year Grants|
|Research Institution||Kanazawa University|
WADA Keishiro Dept.of Biol., Fac.of Sci., Kanazawa Univ. professor, 理学部, 教授 (70028174)
GOLBECK J.h. Dept.of Biochem., Univ.of Nebraska (Lincoln), 生化学, 教授
BLANKENSHIP R.e Dept.of Chem./Biochem., Arizona State Univ. (Tempe), 生化学・化学, 教授
BUCHANAN B.b. Dept.of Plant Biol., Univ.of California (Berkeley), 植物生物学, 教授
TAKAHASHI Yasuhiro Dept.of Biol., Fac.of Sci., Osaka Univ., 理学部, 助手 (10154874)
OHOKA Hirozo Dept.of Biol., Fac.of Sci., Osaka Univ., 理学部, 助手 (30201966)
MATSUBARA Hiroshi Dept.of Biochem., Fac.of Sci., Okayama Science Univ., 理学部, 教授 (00028242)
HOSHINA Satoshi Dept.of Biol., Fac.of Sci., Kanazawa Univ., 理学部, 助教授 (50019486)
|Project Period (FY)
Completed(Fiscal Year 1995)
|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1995 : ¥2,100,000 (Direct Cost : ¥2,100,000)
|Keywords||chloroplasts / plastids / photosystem complex / green sulfur bacteria / electron tranport system / FNR / ferredoxins / thioredoxin|
Regulation mechanism of the electron transport in chloroplasts and the related platids was investigated by the group including three American scientists who are experts in each field. Experiments were planed by focussing on
1) regulation mechanism of electron transport in photosystem I core complex,
2) regulation mechanism of electron transport to NADP^+ or from NADP^+ around ferredoxin in chloroplasts and plastids of non-photosynthetic tissues,
3) regulation mechanism of several proteins by thiol-disulfide exchange reaction which is mediated by thioredoxins,
4) redox regulation by thioredoxin system.
Each electron transferring components in Fe-S type reaction centers from chloroplasts from higher plants, green sulfur bacteria and primitive bacterium Heliobacter mobilis were compared in the aspect of structure and function.
With PCR method cDNA of ferredoxin-NADP oxidoreductase mainly expressed in non-photosynthetic tissues was cloned and compared with other cDNA sequences of FNR from rice and maize.
The new function of thioredoxin h was characterized and the allergenicity in food proteins was clarified to be reduced by thioredoxin.