| Co-Investigator(Kenkyū-buntansha) |
徐 桂雲 中国科学院, 化学研究所, 主席研究員(助教授)
WEBSTER Robert ST.JUDE CHILDREN'S RES.HOSPITAL,DEPT.OF MOLECULAR BIOLOGY AND VIROLOGY, 部長
ITZSTEIN Mar Monash University, Victorian College of P, 教授
WARD Peter A UNIVERSITY OF MICHIGAN,MEDICAL SCHOOL, Medical Schoo, 教授
XU Guiyun ACADEMIA SINICA INSTITUTE OF CHEMISTRY
VON ITZSTEIN Mark MANASH UNIVERSITY VICTORIAN COLLEGE OF PHARMACY
WEBSTER Rob Dept. Molecular Biology and Virology, St., 部長
宮本 大誠 静岡県立大学, 薬学部, 助手 (40222335)
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| Budget Amount *help |
¥8,400,000 (Direct Cost: ¥8,400,000)
Fiscal Year 1996: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1995: ¥4,300,000 (Direct Cost: ¥4,300,000)
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| Research Abstract |
This project is aimed 1) to clarify the function of adhesion molecule which is involved in cell-cell interaction, cell-virus and cell-bacteria adhesion via sugar chains, 2) to develope a "new investigational category of Glycobiology", GLYCOPATHOLY,and also 3) to establish fundamental concepts for the development of new drugs to prevent infectious diseases, inflammation and also metastasis of cancer cells. In this project (1995), we found that sulfated glycoconjugates, such as sulfatide, one of the sulfated
glycosphingolipid is more active ligand rather than sialyl Le x for the L-and P-selections. Sulfatide effctively blocked the L-and P-selectin-dependent, cobra venom-induced rat lung inflammationin, and also CC14-induced rat liver inflammation. This year (1996) we found that (1) sulfated colomic acid, polysialyl chain, strongly inhibit cobra venom induced inflammation (coworked with P.A.Ward), (2) sulfatide bound to human and animal influenza viruses and inhibit the virus infection. (3) We also determined the structure and function of the common receptor sialo-sugar chains which involve the human and animal viral infection (coworked with R.G.Webster, Xu Guiyun, M.von Itzstein). (4) Variation of human and animal influenza A viruses could be occurred by the pressure of antibody and also the structure of the receptor sialo-sugar chains, such as sialyl-Gal linkage (2,3 ; 2,6) and sialic acid molecular species (Neu5Ac ; Neu5Gc) (coworked with R.G.Webster,
Xu Guiyun). (5) A putative ligand for L-and P-selectins inhibited B cell proliferation and Ig production. (6) Sulfatide also blocked the endotoxin shock with a concomitant reduction in TNF-alpha production. Above results may be important for developing a new drugs for inflammation and infectious diseases.
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