| Project/Area Number |
07405044
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
高分子合成
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SUNAMOTO Junzo KYOTO UNIVERSITY,Graduate school of Engineering, Professor, 工学研究科, 教授 (80037811)
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| Co-Investigator(Kenkyū-buntansha) |
OKUMURA Yukihisa Shinshu University, Faculty of Engineering Associate Professor, 工学部, 助教授 (40243042)
AKIYOSHI Kazunari KYOTO UNIVERSITY,Graduate school of Engineering, Associate Professor, 工学研究科, 助教授 (90201285)
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| Project Period (FY) |
1995 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥36,800,000 (Direct Cost: ¥36,800,000)
Fiscal Year 1998: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1997: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1996: ¥11,100,000 (Direct Cost: ¥11,100,000)
Fiscal Year 1995: ¥19,800,000 (Direct Cost: ¥19,800,000)
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| Keywords | Membrane protein / Liposome / cadherin / cell-cell adhesion / O_2-electrode / O_2 uptake / Ganglioside / cell-stimulation / 膜タンパク質移行 / 赤血球細胞膜 / 細胞内カルシウム / T細胞活性化 / 膜タンパク質再構成 / E-カドヘリン / F9細胞 / シアル酸 / T細胞刺激 / 細胞内カルシウム濃度 |
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| Research Abstract |
Transfer of E-cadherin, which is a cell-cell adhesion molecule and expressed on mouse teratocarcinoma F9, from the cell to liposome was first investigated. The lipid composition of liposome and the condition of the incubation with cell were altered, and the transfer of membrane proteins was optimized. For the demonstration of the retransfer of E-cadherin from liposome to another cell, then cells such as Jurkat cell or B 16 melanoma were coincubated with the liposome on which membrane proteins containing E-cadherin was reconstituted by intermembrane protein transfer. Originally these cells do not adhere to F9 cell, however they acquired the adhesion activity to F9 cell after coincubation with the liposome. It was suggested that E-cadherin was transferred from the liposome to other cell membranes keeping its adhesion activity, and meant that the function of cells were successfully modified by the introduction of E-cadherin.
A new method to determine oxygen uptake rate of living cells is described. Two kinds of cultured mammalian cells were incubated in a 2.0 ml vol cuvette, which was equipped with an oxygen electrode as specially designed to measure the oxygen concentration ([02]) in the culture medium. This new method allowed 1) instant determination of the respiratory activity of living cells, and 2) simultaneous monitoring of responses of cell against extracellular stimuli.
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