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Changes in neuroactive substances in a rat model of neuropathic pain and the role of hyperexcitability in neurons of spinal cord-dorsal colum-thalamus pathway

Research Project

Project/Area Number 07457007
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionHYOGO COLLEGE OF MEDICINE

Principal Investigator

NOGUCHI Koichi  HYOGO COLLEGE OF MEDICINE,PROFESSOR, 医学部, 教授 (10212127)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥5,700,000 (Direct Cost: ¥5,700,000)
Keywordsneuropathic pain / spinal dorsal horn / gracile nucleus / Fos / dorsal root ganglion / neuroactive substances / 末梢神経障害
Research Abstract

The aim of this study was to examine the change in mRNA expression of inhibitory amino acid (GABAA and glycine) receptor subunits and neuropeptides in DRG of an experimental rat model of neuropathic pain. Rats received tight ligations of left L5 spinal nerves under deep anesthesia. After confirmation of the presence of neuropathic pain behaviors at 7th postoperative days, L4,5 DRG and spinal cord were dissected out for in situ hybridization and immunohistochemistry. L5 DRG neurons showed the down-regulated expression ofmRNAs of gamma2-GABAA receptor and beta1-glycine receptor subunits. Immunohistochemistry with calcitonin gene-related peptide antibody revealed that increased immunostaining in laminae III-IV of L4-5 spinal cord These data suggest that depressed inhibitory effect of amino acid and increased excitatory effect ofneuropeptide on dorsal horn neurons may contribute to the pathomechanisms of neuropathic pain.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Noguchi K.et al: "Substonce Pinduced by penpleral nerve ingury in primary offevent Sonsory neurons and its effect on dorsal columin nucleus neurons" Journal of Neuroscience. 15. 9633-9643 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kashiba,H. et al: "Co expressin of trk family membors and low‐affinity ncurotrophin receptors in rat dorsal voot ganglion neurons" Molecular Bran Research. 30. 158-164 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] MiKi. K. et al: "CSRP nerease in the rat spinal dusal horn and dorsi/column nucleus following penpheral nerve injury,Up‐repulation" Nearoscience. (in Press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Noguchi, K., Kawai, Y., Fukuoka, T., Senba, E.and Miki, K.: "Substance p induced by peripheral nerve injury in primary afferent sensory neurons and its effect on dorsal column nucleus neurons" J.Neurosci.15. 7633-7643 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kashiba, H., Noguchi, K., Ueda, Y.and Senba, E.: "Coexpression of trk family members and low-affinity neurotrophin receptors in rat dorsal root ganglion neurons" Mol.Brain Res. 30. 158-164 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Miki, K., Fukuoka, T., Tokunaga, A.and Noguchi, K.: "Calcitonin gene-related peptide increase in the rat spinal dorsal horn and dorsal column nucleus following peripheral nerve injury : Up-regulation in a subpopulation of primary afferent sensory neurons" Neuroscience. (In Press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Noguchi K.et al.: "Substance P mdaced by peripheral nerve myury in primsry atterent Sensory neurons and its etfect on dorsal column nucleus newrns" Journal of Newoscience. 15. 7633-7643 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kashiba,H.et al: "Co-expression of tric tamily members and low-olfinity neulbtrophin receptors in rat dorsal voot gaylim reurons" Molecular Bran Research. 30. 158-164 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Miki K.et al: "CGRP mcrease in ete rat spmal cord dorsal horn and dorsal cilnmn nudons follamg penpheral nenemjury : Up-regulation na subpopulation--" Neurosciene. (In Press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Noguchi k. et al: "Substance P induced by peniphoral nerve injury in primary afferant sensory neurms and its effect on dorsal column nuclens ncums." Journal of Neuroscience. 15. 7633-7643 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kashiba, H. et al: "Coexpression of trk family members and low-affinity neuro trophin peceeptors in rot dosal root ganglion neuras" Molecular Bran Reseach. 30. 158-164 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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