Roles of membrane-type matrix metalloproteinase in tissue organization.
| Project/Area Number |
07457057
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Kanazawa University |
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Principal Investigator |
SEIKI Motoharu Cancer Research Institute, Kanazawa University Professor, がん研究所, 教授 (10154634)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Hiroshi Cancer Research Institute, Kanazawa University Assistant professor, がん研究所, 助手 (00115239)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥7,900,000 (Direct Cost: ¥7,900,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥6,100,000 (Direct Cost: ¥6,100,000)
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| Keywords | Matrix metalloproteinas / Membrane-type matrix metalloproteinase / Gelatinase A / TIMP-2 / Mouse embryo / Tissue remodeling / マトリックスメタロプロテイナーゼ / セラチナーゼA / マトリックスメタロプロテアーゼ / 組織構築 / マウス発生過程 / トロフォブラスト |
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| Research Abstract |
Matrix metalloproteinases (MMPs) are pivotal enzymes for tissue remodeling of multicellular organisms by degrading the components of extracellular matrix. Since MMPs are secreted as inactive zymogens (pro-MMPs), they have to be activated in the tissue to function. However little is known about physiological activators in vivo where active tissue remodeling is taking place. We recently identified a membrane-type MMP (MT-MMP) as an activator of pro-MMP-2 (pro-gelatinase A=72-kDa type IV pro-collagenase) that is expressed in invasive tumors and is responsible for degradation of type IV collagen in the basement membrane. In this study we examined whether MT-MMP plays a role in activating pro-MMP-2 during mouse embryogenesis. MT-MMP expression was increased together with that of MMP-2 and its inhibitor TIMP-2 in embryos according to the days after gestation and decreased with maturation after birth.MT-MMP transcripts and the product were localized in the cells of the occificating tissues where both MMP-2 and TIMP-2 were detected. Activated MMP-2 was detected in the lysate prepared from the microdissected tissue where expression of the three genes were observed. Activated MMP-2 was propotional to the expression of MMP-2 and MT-MMP.Thus the results indicate that MMP-2 and its activator MT-MMP is a widely used system for organisms to initiate tissue remodeling on the cell surface.
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Report
(3 results)
Research Products
(9 results)
