| Project/Area Number |
07457117
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Yamagata University School of Medicine |
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Principal Investigator |
SUZUKI Tsuneo Yamagata University School of Medicine, Department of Forensic Medicine, Professor, 医学部, 教授 (70004588)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Hiroshi Yamagata University School of Medicine, Department of Forensic Medicine, Assista, 医学部, 助手 (10142217)
UMETSU Kazuo Yamagata University School of Medicine, Department of Forensic Medicine, Assista, 医学部, 助教授 (10091828)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1995: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | Sudden death / ventricular fibrilation / Myocardial infarction / Luxol fast blue staining / ルクソ-ルファストブルー染色 |
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| Research Abstract |
To Investigate the relatlonshIp between sudden death and the time elapsed after the onset of myocardial infarction and to find a suitable staining method for detection of acute myocardial Infarction, an experimental model of acute myocardial ischemia was made with mongrel dogs by ligating anterior descending branch of left coronary artery. The heart was given electrical simulation at the vulnerable period of the cardiac cycle and the ventricular fibrillation threshold was estimated. Ventricular fibrillation threshold decreased to about 20 % of those of the control hearts 15-30 mm after the coronary artery ligation. Then ii increased with the time elapsed after the coronary ligation and showed almost same values as those of control hearts 4 h after the coronary ligation. The ischemic hearts were fixed with 10 % formalin solution. The sections from the ischemic heart muscle were stained with Luxol Fast Blue (LFB) solution for 24 h and were differentiated for 1-3 min in several changes of 75 % alcohol to a faint dove-colored blue (a newly modified LFB staining). The newly modified LFB staining revealed the myocardial ischemic changes 15-30 min after the coronary ligation. The result suggested that sudden death from ventricular fibrillation is the aptest to occur 15-30 min after the onset of myocardial infarction and the newly modified LFB staining Is useful for detecting such very early myocardial infarction.
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