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Molecular biological analysis of the mechanism of acute renal failure

Research Project

Project/Area Number 07457242
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionKumamoto University

Principal Investigator

TOMITA Kimio  Kumamoto University, School of Medicine, Professor, 医学部, 教授 (40114772)

Co-Investigator(Kenkyū-buntansha) KITAMOTO Yasunori  Kumamoto University, School of Medicine, Associate Professor, 医学部, 講師 (80195297)
有馬 省哉  熊本大学, 医学部・附属病院, 助手 (00244129)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1996: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1995: ¥3,200,000 (Direct Cost: ¥3,200,000)
Keywordsacute renal failure / gene / glomerulus / tubules / endothelin / receptor
Research Abstract

We investigated the expression and regulation of endothelin-1 protein and mRNA,endothelin converting enzyme protein and mRNA,A type endothelin receptor mRNA,B type endothelin receptor mRNA in ischemic and cyclosporine-induced acute renal failure. Endothelin-1 mRNA started to increase from 30 minutes to several hours after ischemic insult or cyclosporine administration. A type endothelin receptor mRNA decreased in both types of acute renal failure. B type endothelin receptor mRNA increased in ischemic acute renal failure. However, it decreased in cyclosporine-induced acute renal failure. Endothelin converting enzyme protein and mRNA decreased in cyclosporine-induced acute renal failure. To elucidate the mechanism of inhibition of endothelin converting enzyme protein and mRNA,regulation of endothelin converting enzyme was investigated in cultured endothelial cells. Endothelin-1 has an inhibitory effect on endothelin converting enzyme protein and mRNA through B type endothelin receptor. These data suggests that impairments of renal function may be protected by the decrease in A type endothelin receptor and increase in B type endothelin receptor in ischemic acute renal failure. As for cyclosporine-induced acute renal failure, impairments of renal function may be protected by the decrease in A type endothelin receptor and decrease in endothelin converting enzyme through B type endothelin receptor.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] H.Nonoguchi,et al: "Immunohistochemical localization of V2 vasopressin receptor along the nephron and functional role of luminal V2 receptor in terminal inner medullary collecting ducts." J.Clin.Invest.96. 1768-1778 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshio Terada,et al: "Sequential activation of MAP kinase cascade by angiotensin II in opossum kidney cells." Kidney Int.48. 1801-1809 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Yamada,et al: "AVP inhibits EGF-stimulated MAP kinase cascade in Madin-Darby canine kidney cells." Kidney Int.48. 745-752 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Terada,et al: "Presence and regulation of Raf-1-K (kinase),MAPK-K,MAP-K,and S6-K in rat nephron segments." J.Am.Soc.Nephrol.6. 1565-1577 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Owada,et al: "Microlocalization and effects of adrenomedullin in microdissected nephron segments and in cultured mesangial sells of the rat." Am.J.Physiol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Kitamoto,et al: "Vascular endothelial growth factor (VEGF) is an essential molecule for mouse kidney development : glomerulogenesis and nephrogenesis." J.Clin.Invest.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Nonoguchi: "Immunohistochemical localization of V2 vasopressin receptor along the nephron and functional role of luminal V2 receptor in terminal inner medullary collecting ducts." J.Clin.Invest.96. 1768-1778 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshio Terada: "Sequential activation of MAP kinase cascade by angiotensin II in opossum kidney cells." Kidney Int.48. 1801-1809 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Yamada: "AVP inhibits EGF-stimulated MAP kinase cascade in Madin-Darby canine kidney cells." J.Am.Soc.Nephrol. 6. 1565-1577 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Terada: "Presence and regulation of Raf-1-K (kinase), MAPK-K,MAP-K,and S6-K in rat nephron segments." Am.J.Physiol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Owada: "Microlocalization and effects of adrenomedullin in microdissected nephron segments andin cultured mesangial cells of the rat." J.Clin.Invest.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Kitamoto: "Vascular endothelial growth factor (VEGF) is an essential molecule for mouse kidney development : glomerulogenesis and nephrogenesis." Am.J.Physiol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Kitamoto,H.: "Vascular endothelial growth factor (VEGF) is an essential molecule for mouse kidney development : glomerulogenesis and nephrogenesis." J.Clin.Invest.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] A.Owada,: "et al Microlocalization and effects of adrenomedullin in microdissected nephron segments and in cultured mesangial cells of the rat." Am.J.Physiol.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Nonoguchi,: "Role of urinary arginine vasopressin in the sodium excretion in patients with chronic renal failure." Am.J.Med Sci. 312. 195-201 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Disashi,: "Magnesium metabolism in hyperthyroidism." Endocrine J.43. 397-402 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Nonoguchi,: "Immunohistochemical localization of V2 vasopressin receptor along the nephron and functional role of luminal V2 receptor in terminal inner medullary collecting ducts." J.Clin.Invest.96. 1768-1778 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshio Terada,: "Sequential activation of MAP kinase cascade by angiotensin II in opossum kidney cells." Kidney Int.48. 1801-1809 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Yamada,: "AVP inhibits EGF-stimulated MAP kinase cascade in Madin-Darby canine kidney cells." Kidney Int.48. 745-752 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Yang,: "Expression of mRNAs for different types of IP3 receptors in rat kidneys." Am.J.Physiol.268. F1046-F1052 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Terada,: "Presence and regulation of Raf-1-K (kinase) ,MAPK-K,MAP-K,and S6-K in rat nephron segments." J.Am.Soc.Nephrol.6. 1565-1577 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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