|Budget Amount *help
¥6,200,000 (Direct Cost : ¥6,200,000)
Fiscal Year 1997 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 1996 : ¥1,600,000 (Direct Cost : ¥1,600,000)
Fiscal Year 1995 : ¥3,900,000 (Direct Cost : ¥3,900,000)
1) We have attempted to detect the presence and expression of several virus genes in human esophageal carcinoma cells using a pannel of 15 sample cells consisting of 8 cell lines and 7 nude mouse-transplantable esophageal caricinomas which were established for culture in vitro and trasplant in nude mice in our labratory, respectively. As for virus DNA probes for the hybridazation, human papilloma viruses (types 1a, 2a, 4,6,11,16 and 18), EBV,SV40 and HBV,obtained from Japanese Cancer Research REsources Bank, were used. However, the presence and expression of these virus genes were failed to detect in the used esophageal carcinoma cells.
2) Using the same cell pannel, the relation between temperature sensitivity on the hyperthermia and number ofmitochondoria, succinate dehydrogenase (SD) activity or fluorescence intensity (FI) after staining with Rhodamine 123 has been examined. As a result, FI shows the best correlation with sensitivity on hyperthermia among these mitochondorial markers
3) To further studies, esophageal carcinoma tissues haved been collected from several patients and established as culturable cell lines and transplantable.carcinomas in nude mice. Sera of the patients have also been collected and stored.