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Experimental analysis of lung cancer induced by radon exposure and smoking

Research Project

Project/Area Number 07458130
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境影響評価(含放射線生物学)
Research InstitutionShiga University of Medical Science

Principal Investigator

IKEBUCHI Makoto  School of Medicine, Shiga University of Medical Science, Research Associate, 医学部, 助手 (10121550)

Co-Investigator(Kenkyū-buntansha) KIMURA Hiroshi  School of Medicine, Shiga University of Medical Science, Full Professor, 医学部, 教授 (00110560)
青山 喬  滋賀医科大学, 医学部, 教授 (60039526)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥3,300,000 (Direct Cost: ¥3,300,000)
Keywordsradon / radon progeny / radon exposure system / rats / lung fibroblasts / micronuclei / biological effects / superoxide dismutase / ラット / 突然変異 / 肺繊維芽細胞 / 気管上皮細胞 / DNA配列
Research Abstract

Experimental animal studies are thought to be useful for estimating risks of radon exposure. Such studies, however, had not done in Japan because of lack of radon exposure system. We developed a radon exposure system with 0.215 m^3 chamber in which 3 rats can be exposed. Radon concerntration measured by PICO-RAD system was fairly constant during exposure whereas the concentration of radon progeny monitored by filter-trapping method decreased. After exposure to radon, rats were sacrificed , and the frequency of micronuclei induced in lung fibroblasts was determined. The micronucleous frequency was related to the concentration of radon rather than that of radon progeny. When an average frequency of micronuclei (about 3%) was extrapolated to dose-response curves for X-ray induced micronuclei, the dose received by lung fibroblasts was found to correspond to 0.2-0.3 Gy of X-rays. The micronucleous frequency can be used as biological dosimeter. We tested for the activity of superoxide dismutase (SOD) in tissues using an electron spin resonance spectrometer. SOD activity was increased in kidney, liver and spleen after 4 h exposure, whereas it decreased after 16 h exposure, suggesting that this stimulating effect could last for a short time. The enhancement in SOD activity has been reported to occur after exposure to several Gy of X-rays. Compared with X-rays, the exposure of radon gave biological effects at lower absorbed doses. These findings would be helpful, when the experiments to determine the in vivo frequencies of cell transformation and 6-thioguanine resistant mutation are advanced for estimating risks of radon.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Ikebuchi, M: "Are three forms of potentially lethal damage expressed after X irradiation by treatment with hypertonic solutions in Chinese hamster V79 cells?" Radiat. Res.141. 19-27 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kimura, H: "Cultured cells from a severe combined immunodeficlent mouse have a slower than normal rate of repair of potentially lethal damage sensitive to hypertonic treatment" Radiat. Res.142. 176-180 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nyaruba, M. M: "Increase of bone mineral content in rat tibia after irradiation" Osteoporosis Japan. 3. 110-113 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ma, J: "Effect of radon exposure on superoxide dismutase (SOD) activity in rats." J. Radiat. Res.37. 12-19 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 池淵 誠: "PLDRはいくつあるか?" 放射線生物研究. 31. 191-205 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kimura, H: "The scid mutation does not affect slowley repairing potentially lethal damage that is sensitive to 0.23 M NaCl" J. Radiat. Res.37. 247-255 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ikebuchi, M.et al.: "Are three forms of potentially lethal damage expressed after X irradiation by treatment with hypertonic solutions in Chinese hamster V79 cells?" Radiat.Res.141. 19-27 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kimura, H.et al.: "Cultured cells from a severe combined immunodeficient mouse have a slower than normal rate of repair of potentially lethal damage sensitive to hypertonic treatment" Radiat. Res.142. 176-180 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nyaruba, M.M.et al.: "Increase of bone mineral content in rat tibia after irradiation" Osteoporosis Japan. 3. 110-113 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ma, J.et al.: "Effect of radon exposure on superoxide dismutase (SOD) activity in rats." J.Radiat.Res.37. 12-19 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ikebuchi, M.et al.: "How many potentially lethal damage are there?" Housyasen Seibutsu kenkyu. 31. 191-205 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kimura, H.et al.: "The scid mutaion does not affect slowly repairing potentially lethal damage that is sensitive to 0.23 M NaCl" J.Radiat.Res.37. 247-255 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ikebuchi,M: "Are three forms of potentially lethal damage expressed after X irradiation by treatment with hypertonic solutions in Chinese hamster V79 cells?" Radiat.Res.141. 19-27 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kimura,H: "Cultured cells from a severe combined immunodeficient mouse have a slower than normal rate of repair of potentially lethal damage sensitive to hypertonic treatment" Radiat.Res.142. 176-180 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nyaruda,M.M: "Increase of bone mineral content in rat tibia after irradiation" Osteoporosis Japan. 3. 110-113 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ma,J: "Effect of radon exposure on superoxide dismutase(SOD)activity in rats." J.Radiat.Res.37. 12-19 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 池淵 誠: "PLDRはいくつあるか?" 放射線生物研究. 31. 191-205 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kimura,H: "The scid mutation does not affect slowley repairing potentially lethal damage that is sensitive to 0.23 M NaCl" J.Radiat.Res.37. 247-255 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Jizeng Ma et.al.: "Effect of radon exposure on superoxide dismutase(SOD)activity in rats" Journal of Radiation Research. 37. (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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