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Construction of externally controllable gene promoters in plants

Research Project

Project/Area Number 07554044
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 植物生理
Research InstitutionThe University of Tokyo

Principal Investigator

WATANABE Akira  The University of Tokyo, Graduate School of Science, Professor, 大学院・理学系研究科, 教授 (70023471)

Co-Investigator(Kenkyū-buntansha) ITO Masaki  The University of Tokyo, Graduate School of Science, Research Associate, 大学院・理学系研究科, 助手 (10242851)
FUJIMURA Tatsuhito  Tsukuba University, Graduate School of Biosystem, Professor, 農林工学系バイオシステム研究科, 教授
MATSUOKA Makoto  Nagoya University, BioScience Center, Professor, 生物分子応答研究センター, 教授 (00270992)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥20,000,000 (Direct Cost: ¥20,000,000)
Fiscal Year 1997: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1996: ¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1995: ¥11,300,000 (Direct Cost: ¥11,300,000)
KeywordsPlant genes / Gene promoter / Gene expression / Arabidopsis thaliana / Sugar starvation / Tobacco BY-2 cells / Rice plant / Thiamin / 遺伝子プロモーター / チアミン抑制遺伝子 / 植物遺伝子プロモーター / 糖飢餓応答遺伝子 / 形質転換植物 / タバコ培養細胞 / 遺伝子導入 / 窒素栄養 / サリチル酸
Research Abstract

This research has been conducted to obtain a good externally controllable promoter to be used in the analysis of gene functions in plants. To do this we first isolated cDNA clones for genes that are expressed in plants under very specific conditions. These conditions included those deprivation of thiamine from culture media for tobacco BY-2 cells, dark treatment of Arabidopsis leaves and application of salicylic acid to tobacco plants. We could isolate several clones by a direct-display PCR procedure, but the expression of the genes complimentary to those cDNA sequences were not very specific, ie.the transcripts were found even in the presence of the vitamin. We were successful to find several genes that are expressed by a manner very specific to the second conditions. Three out of more than a dozen genes found to be dark-inducible were selected for rapid and specific expression after careful northern hybridization analysis. Their promoter regions were isolated and fused with a reporter gene (firefly luciferase). The fused genes were introduced into cultured BY-2 cells and looked at the expression after deprivation of sugar from the culture media, because we noticed in the northern analysis that the dark-induced expression was abolished if the leaves were fed with sucrose at 10mM.The transformed cells were very sensitive to sugar starvation in respect to the expression of the reporter gene. As the expression is very rapid, strong and reversible, and the control the promoter can be done by a very mild and naturally occurring stimuli, it appears promising as a tool for the analysis of functions of genes not favorable for normal growth, and for agricultural use for crop improvement as well.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (48 results)

All Other

All Publications (48 results)

  • [Publications] Ito, M.: "Call-Cycle-regulated transcritption of A-and B-type plant cyclin genes in synchronous cultures." Plant J.11. 983-992 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Watanabe, A.: "Internal Gln/Gln ratio as a potential regulatory parameter for the expression of a cytosolic glutamine synthetase gene of radish in cultured cells." Plant Cell Physiol.38. 1000-1006 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Smith, M.W.: "Plant21D7 protein, a nuclear antigen associated with cell division,is a component of the 26S proteasome." Plant Physiol.113. 281-291 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shimada, Y: "A protein encoded by dinl,a dark-inducible and senescence-associated gene of radish,can be imported by isolated chloroplasts and has sequence similarity to sulfide dehydrogenase and other small stress proteins." Plant Cell Physiol.39. 139-143 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Imaizumi, N.: "Characterization of the gene for pyruvate, orthophosphate dikinase from rice, a C3 plant,and a comparison of structure and expression between C3 and C4 genes for this protein." Plant Mol.Biol.34. 701-716 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Makino, A.: "Does decrease in ribulose-1,5 bisphosphate carboxylase by antisense RbcS lead to a higher N-use efficiency of photosynthesis under conditions of saturating CO_2 end light in rice plants?" Plant Physiol.114. 483-491 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tamaoki, M.: "Isolation and characterization of cDNA clones with enhanced expression in tobacco plants expressing the rice homeobox gene, OSH1." Plant Cell Physiol.38. 638-642 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tamaoki, T.: "Dorsoventral pattern formation of tobacco leaf involves spatial expression of a tobacco homeobox gene, NTH15." Genes Genet.syst.72. 1-8 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sanmiya, K.: "Cloning of a cDNA that cncodes farnesyl diphosphate synthase and the blue-light-induced expression of the corresponding gene in the leaves of rice plants." biochim. Biophys.Acta. 1350. 240-246 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tamaoki, M.: "Ectopic expression of a tobacco homeobox gene,NTH15,dramatically alters leaf morphology and hormone levels in transgenic tobacco." Plant Cell Physiol.38. 917-927 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akagi, H.: "Highly polymorphic microsatellites of rice consist of AT repeats, and a classification of closely related cultivars with these microsatellite loci." Theor.Appl.Genet.94. 61-67 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kodama, H.: "Structure, chromosomal location and expression of a rice gene encoding the microsome w-3 fatty acid desaturase." Plant Mol.Biol.33. 493-502 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito, M.: "Cell-cycle-regulated transcription of A-and B-type plant cyclin genes in synchronous cultures." Plant J.11. 983-992 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Watanabe, A.: "Internal Gln/Glu ratio as a potential regulatory parameter for the expression of a cytosolic glutamine synthetase gene of radish in cultured cells." Plant Cell Physiol.38. 1000-1006 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Smith, M.W.: "Plant 21D7 protein, a nuclear antigen associated with cell division, is a component of the 26S proteasome." Plant Physiol.113. 281-291 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shimada, Y.: "A protein encoded by din1, a dark-inducible and senescence-associated gene of radish, can be imported by isolated chloroplasts and has sequence similarity to sulfide dehydrogenase and other small stress proteins." Plant Cell Physiol.39. 139-143 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Imaizumi, N.: "Characterization of the gene for pyruvate, ortho-phosphate dikinase from rice, a C3 plant, and a comparison of structure and expression between C3 and C4 genes for this protein." Plant Mol.Biol.34. 7001-716 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Makino, A.: "Does decrease in ribulose-1,5 bisphosphate carboxylase by antisense RbcS lead to a higher N-use efficiency of photosynthesis under conditions of saturating CO_2 and light in rice plants?" Plant Physiol.114. 483-491 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tamaoki, M.: "Isolation and characterization of cDNA clones with enhanced expression in tobacco plants expressing the rice homeobox gene, OSH1." Plant Cell Physiol.38. 638-642 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tamaoki, M.: "Dorsoventral pattern formation of tobacco leaf involves spatial expression of a tobacco homeobox gene, NTH15." Genes Genet.Syst.72. 1-8 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sanmiya, K.: "Cloning of a cDNA that encodes farnesyl diphosphate synthase and the blue-light-induced expression of the corresponding gene in the leaves of rice plants." Biochim.Biophys.Acta. 1350. 240-246 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tamaoki, M.: "Ectopic expression of a tobacco homeobox gene, NTH15, dramatically alters leaf morphology and hormone levels in transgenic tobacco." Plant Cell Physiol.38. 917-927 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akagi, H.: "Highly polymorphic microsatellites of rice consist of AT repeats, and a classification of closely related cultivars with these microsatellite loci." Theor.Appl.Genet.94. 61-67 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kodama, H.: "Structure, chromosomal location and expression of a rice gene encoding the microsome w-3 fatty acid desaturase." Plant Mol.Biol.33. 493-502 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito,M.: "Cell-cycle-regulated transcritption of A-and B-type plant cyclin genes in synchronous cultures." Plant J.11. 983-992 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Watanabe,A.: "Internal Gln/Glu ratio as a potential regulatory parameter for the expression of a cytosolic glutamine synthetase gene of radish in cultured cells." Plant Cell Physiol.38. 1000-1006 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Smith,M.W.: "Plant 21D7 protein,a nuclear antigen associated with cell division,is a component of the 26S proteasome." Plant Physiol.113. 281-291 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shimada,Y.: "A protein encoded by dinl,a dark-inducible and senescence-associated gene of radish,can be imported by isolated chloroplasts and has sequence similarity to sulfide dehydrogenase and other small stress proteins." Plant Cell Physiol.39. 139-143 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Imaizumi,N.: "Characterization of the gene for pyruvate,orthophosphate dikinase from rice,a C3 plant,and a comparison of structure and expression between C3 and C4 genes for this protein." Plant Mol. Biol.34. 701-716 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Makino,A.: "Does decrease in ribulose-1,5 bishosphate carboxylase by antisense RbcS lead to a higher N-use efficency of photosynthesis under conditions of saturating CO_2 and light in rice plant?" Plant Physiol.114. 483-491 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tamaoki,M.: "Isolation and characterization of cDNA clones with enhanced expression in tobacco plants expressing the rice homeobox gene,OSH1." Plant Cell Physiol.38. 638-642 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tamaoki,T.: "Dorsoventral pattern formation of tobacco leaf involves spatial expression of a tobacco homeobox gene,NTH15." Genes Genet.Syst.72. 1-8 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sanmiya,K.: "Cloning of a cDNA that encodes farnesyl diphosphate synthase and the blue-light-induced expression of the corresponding in the leaves of rice plants." Biochim.Biophys.Acta. 1350. 240-246 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tamaoki,M.: "Ectopic expression of a tobacco homeobox gene,NTH15,dramatically alters leaf morphology and hormone levels in transgenic tobacco." Plant Cell Physiol.38. 917-927 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Akagi,H.: "Highly polymorphic microsatellites of rice consist of AT repeats,and a classification of closely related cultivars with these microsatellite loci." Theor.Appl.Genet.94. 61-67 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kodama,H.: "Structure,chromosomal location and expression of a rice gene encoding the microsome w-3 fatty acid desaturase." Plant Mol.Biol.33. 493-502 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ito,M.,et al.: "Cell-cycle regulated trancription of A-and B-type plant cyclin genes in synchronous cultures" Plant J.印刷中 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sato,Y.,et al.: "Abnormal cell divisions in leaf primordia caused by the expression of the rice homeobox gene OSH1 lead to altered morphology of leaves in transgenic tobaco" Mol.Gen.Genet.251. 13-22 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sato,Y.,et al.: "A rice homeobox gene, OSH1,is expressed prior to organ differentiation in a specific region during early embryogenesis" Proc.Natl.Acad.Sci.U.S.A.93. 8117-8122 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Gallusci,P.,et al.: "Regulation of cytosolic pyruvate, orthophosphate dikinase expression in developing maize endosperm" Plant Mol.Biol.31. 45-55 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ku,M.,et al.: "Evolution and expression of C4 photosynthesis genes" Plant Physiol.111. 949-957 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Wu,G.and Watanabe,A.: "Import of modified D1 portein and its assembly into photosystem II by isolated chloroplasts" Plant Cell Physiol.38(印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sanmiya,K.,et al.: "Cloning of a cDNA that encodes farnesyl diphosphate synthetase and the blue light-induced expression of the corresponding gene in the leaves of rice plants" Biochim.Biophys.Acta. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsuoka, M.: "Expression of nice OSH1 gene is localized indeveloping vaseular strands and its setepic expression in transgenle rice causes altered morphology" Plant Cell Reports. 14. 555-559 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tamaoki, M.: "Alternative RNA products from a rice homeobox gene" Plant J.7. 927-938 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Matsuoka, M.: "The gene for pynuvate, orthophosphate aikinase in C4 plants: structure regulation and evohtion" Plant Cell Physiol. 36. 937-943 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sato, Y.: "Abnormal cell divisions in leaf primondia cansed by the expression of the rice homeobox gene OSH1 lead to altened" Mol. Gen. Genet.in press (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 渡辺 明夫: "緑葉老化における窒素利用プログラム" 組織培養. 22. 15-22 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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