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Humanization of mouse anti-human IL-8 antibody and development of anti-inflammatory agent against cytokine regulatory factor, NFkB

Research Project

Project/Area Number 07557031
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Immunology
Research InstitutionUniversity of Tokyo (1996-1997)
Kanazawa University (1995)

Principal Investigator

MATSUSHIMA Kouji  University of Tokyo, Graduate School of Medicine, Development Preventive Medicine, Professor, 大学院・医学系研究科, 教授 (50222427)

Co-Investigator(Kenkyū-buntansha) YAMAZAKI Tatsumi  Chugai Pharmaceutical Company, Chief Director, 所長(研究職)
向田 直史  金沢大学, がん研究所, 助教授 (30182067)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥11,500,000 (Direct Cost: ¥11,500,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1995: ¥5,500,000 (Direct Cost: ¥5,500,000)
Keywordsinterleukin-8 / anti-inflammatory agent / acute inflammation / anti-interleukin-8 antibody / humanization / NF-kB / IkB-bound kinase / phosphorylation / ケモカイン / 白血球 / 転写因子 / NFkB / IkBα / 単球走化活性化因子 / 抗体
Research Abstract

IL-8 is essentially involved in neutrophil-dependent tissue damage in acute inflammatory reactions. We established the humanized mouse anti-human IL-8 by mean of complementarity determining region grafting and succeeded to purify the large amount of this antibody. For the application of this antibody on various clinical condition, we established various acute inflammatory ananimal models. Especially, we have established preclinical condition animal models such as acute respiratory distress syndrom-like lung injury and brain reperfusion injury. In these models, we showed that anti-IL-8 antibody treatment almostly prevented these injury. These results strongly suggest the possibility of clinical application of humarized anti-human IL-8 antibody against acute inflammatory dieases.
We have identified a kinase in cell extracts from the LPS-stimulated human monocytic cell line, THP-1, that specifically bind and phosphorylates IkBa. LPS-stimulation transiently enhanced the IkBa-bound kinase activity in THP-1 cells. Mutation analysis of IkBa and competition experiments with the synthetic peptides identified major phosphorylation site by the bound kinase as Ser and Thr residues in the C-terminal acidic domain of IkBa. Moreover, this IkBa-boundkinase is novel kinase but not Ikka, b which are related to signal pathway of TNF-a and IL-1. So that we try to purify the kinase SDS-PAGE analysis showed that the kinase. is 40 Kd. We are now analyzing the amino acid squence of the sample and trying to clone the gene.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (94 results)

All Other

All Publications (94 results)

  • [Publications] Kuno, K.et al.: "Identification of an IkBa-associated protein kinase in a human cell lineand determination of its phosphorylation sites on IkBa." J.Biol.Chem.270. 27914-27919 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishikawa, Y.et al.: "Establishment of lipopolysaccharide-dependent nulear factor-kB activation in a cell-free system." J.Biol.Chem.270. 4158-4164 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mukaida, N.et al.: "Novel insight into molecular mechanism of endotoxin shock" J.Leukocyte Biol.59. 145-151 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yokoi, K.et al.: "Prevention of endotoxemia-induced acute respiratory distress syndrome-like lung injury in rabbits by a monoclonal antibody to IL-8." Lab.Invest.76. 375-384 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumoto, T.et al.: "Prevention of cerebral edema andinfarct in reperfuion injury by an antibody to interleukin-8." Lad.Invest.77. 119-125 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Khabar, KSA.et al.: "The α chemokine,interleukin 8,inhibits the antiviral action of interferon α." J.Exp.Med.186. 1077-1085 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumoto, T.et al.: "Rivotal role of interleukin-8(IL-8)in acute inflammation (Invited Review)" J.Leukocyte Biol.62, 7(581-587) (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mukaida, N.et al.: "Cytokine Growth Fact.Rev." Interleukin-8 and monocyte chemotactic and activating factor (MCAF/MCP-1),chemokines essentially involved in inflammatory and immune reactions.(in press), (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kuno, K., Ishikawa, Y., Ernst, M.K., Ogata, M., Rice, N.R., Mukaida, N., and Matsushima, K.: "Identification of a IkBa-associated protein kinase in a human cell line and determination of its phosphorylation sites on IkBa" J.Biol.Chem.270. 27914-2791 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishikawa, Y., Mukaida, N., Kuno, K., Rice, N., Okamoto, S., and Matsushima, K.: "Establishment of lipopolysaccharide-dependent nuclear factor-kB activation in a cell-free system." J.Biol.Chem.270. 4158-4164 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mukaida, N., Ishikawa, Y., Ikeda, N., Fujioka, N., Watanabe, S., Kuno, K., and Matsushima, K.: "Novel insight in to molecular mechanism of endotoxin shock." J.Leukocyte.Biol. 59. 145-151 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yokoi, K., Mukaida, N., Harada, A., Watanabe, Y., Matsushima, K.: "Prevention of endotoxemia-induced acute respiratory distress syndrome-like lung injury in rabbits by a monoclonal antibody to IL-8" Lab.Invest.76. 375-384 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumoto, T., Yokoi, K., Mukaida, N., Harada, A., Yamashita, J., Watanabe, Y., and Matsushima, K.: "Prevetion of cerebral edema and infarct in cerebral reperfusion injury by an antibody to interleukin-8" Lab.Invest.62. 119-125 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Khabar, K.S., Al-Zoghaibi, F., Al-Ahdal, M.N., Murayama, T., Dhalla, M., Mukaida, N., Taha, M., Al-Sedairy, S.T., Siddiqui, Y., Kessie, G., and Matsushima, K.: "The alpha chemokine, interleukin 8, inhibits the antiviral action of interferon alpha." J.Exp.Med.186. 1077-1085 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumoto, T., Yokoi, K., Mukaida, N., Harada, A., Yamashita, J., Watanabe, Y., and Matsushima, K.: "Pivotal role of interleukin-8 in the acute respiratory distress syndrome and cerebral reperfusion injury." J.Leukocyte.Biol.62. 581-587 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mukaida, N., Harada, A., and Matsushima, K.: "Interkeukin-8 and monocyte chemotactic and activating factor (MCAF/MCP-1), chemokines essentially involvedin inflammatory and immune reaactions"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Cytokine Growth Factor.Rev.(1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kuno,K.et al.: "Identification of an IkBa-associated protein kinase in a human cell line and determination of its phospharylation sites on IkBa." J.Biol.Chem.270. 27914-27919 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ishikawa,T.et al.: "Establiahment of lipopolysaccharide-dependent nulear factor-KB activation in a cell-free system." J.Biol.Chem.270. 4158-4164 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mukaida,N.et al.: "Novel insight into molecular mechanism of endotoxin shock" J.Leukocyte Biol.59. 145-151 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yokoi,K.et al.: "Prevention of endotoxemia-induced acute respiratory distress syadrome-like long injury in rabbits by a monoclonal antibody to IL-B." Lab.Invest.76. 375-384 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsumoto,T.et al.: "Prevention of cerebral edema and infarot in reperfuion injury by an antibody to interleukin-8." Lab.Invest.77. 119-125 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Khabar,KSA.et al.: "The alpha chemokine,interleukin8,inhibits the antiviral action of interferon alpha." J.Exp.Med.186. 1077-1085 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsumoto,T.et al.: "Pivotal role of interleukin-8 (IL-8) in acute inflammation (Invited Review)" J.Leukocyte Biol.62, 7(581-587) (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mukaida,N.et al.: "Cytokine Growth Fact.Rev." Interleukin-8 and momocyte chemotactic and activating factor (MCAF/MCP-i),chenokines essentially involved in inflemmatory and immone reactions. (in press), (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wada,T.,Yokoyama,H.and Matsushima,K.et al.: "Intervention of crescentic glomerulonephritis in rats by administering antibodies against monocyte chemotactic and activating factor (MCAF)." FASEB J.10巻. 1418-1425 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yano,S.,Yanagawa,H.,and Matsushima,K.et al.: "Th2 cytokines differently regulates monocyte chemoattractant protein-1 (MCP-1) production by human peripheral blood monocytes and alveolar macrophages." J.Immunol.157巻. 2660-2665 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shono,T.,Ono.and Matsushima,K.et al.: "Involvement of the transcription factor NF-kB in tubular morphogenesis of human microvascular endothelial cells by oxidative stress." Mol.Cell.Biol.16巻. 4231-4239 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mukaida,N.,Ishikawa,Y.,and Matsushima,K.et al.: "Novel insight into molecular mechanism of endotoxin shock." J.Leukocyte Biol.59巻. 145-151 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Harada,A.,Mukaida,N.,and Matsushima,K.: "IL-8 as a novel target for intervention therapy in acute inflammatory diseases." Moi.Med.Today. 2巻. 482-489 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kuno,K.,Kanada,N.,and Matsushima,K.et al.: "Molecular cloning of a gene encoding a new type of metalloproteinase-disintegrin family protein with thrombospondin motifs as an inflammation associated gene." J.Biol.Chem.272巻. 556-562 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Harada,A.,Mukaida,N.,and Matsushima,K.: "Use of blocking antibodies as probes for in vivo functions of chemokines.In A Companion to Methods in Enzymology,vol.10.Sozzani,S.(ed.)" Academic Press,New York,NY,U.S.A., 166-174 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Harada,A.,Mukaida,N.,and Matsushima,K.: "The role of chemokines in ischemia and reperfusion injury.In Chemokines in Diseases.A.Koch and R.M.Strieter(eds.)." R.G.Landers Company,Austin,Texas,USA, 179-193 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Okamoto,S.-i.: "The interleukin-8 AP-1 and kB-like sites are genetic end targets of FK506-sensitive pathway accompanied by calcium mobilization" J. Biol. Chem.269. 8582-8589 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mukaida,N.: "Novel mechanism of glucocorticoid-mediated gene repression: Nuclear factor-kB is target for glucocorticoid-mediated interleukin 8 gene repression" J. Biol. Chem.269. 13289-13295 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakano,Y: "Protection against lethal bacterial infection in mice by monocyte-chemotactic and -activating factor(MACF)." Infect. Immun.62. 377-383 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Murakami,S.: "Transactivation of human hepatitis B virus X protein, HBx, operates through a mechanism distinct from protein kinase C and okadaic acid activation pathway." Virology. 199. 243-246 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Asano,T.: "Liposomal muramyl tripeptide up-regulates interleukin-1 alpha, interuleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 gene expression in human monocytes." J. Pharmacol. Exp. Ther.268. 1032-1039 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kuno,K.,: "Acid sphingomyelinase is not essential for the IL 1 and tumor necrosis factor receptor signaling pathway leading to NFkB activation." Int. Immunol.6. 1269-1272 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kuno,K.: "The IL 1 receptor signaling pathway" J. Leukocyte Biology. 56. 542-547 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Endo,H.: "Experimental arthritis induced by continuous infusion of IL-8 into rabbit knee joints." Clin. Exp. Immunol.96. 31-35 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Harada,A.: "Cloning of a cDNA encoding a mouse homolog of the interleukin-8 receptor." Gene. 142. 297-300 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Deleuran,B.: "Localisation of interleukin 8 in the synovial membrane, cartilage-pannus junction and chondrocytes in rheumatoid arthritis. Scand." Scand. J. Rheumatol.23. 2-7 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Asano,T.: "Liposome-encapsulated muramyl tripeptide up-regulates monocyte chemotactic and activating factor gene expression in human monocytes at the transcriptional and post-transcriptional levels." Cancer Immunology and Immunotherapy. 38. 16-22 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Oliveira,I.C.: "Transcriptional inhibition of the interleukin-8 gene by interferon is mediated by the NF-kB site." Mol. Cell Biol.14. 5300-5308 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Akahoshi,T.: "Essential involvement of interleukin-8 in neutrophil recruitment in rabbits with acute experimental arthritis induced by lipopolysaccharide and interleukin-1." Lymphokine Cytokine Res.13. 113-116 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Itoh,R.: "Interleukin-8 in Behcet's Disease." J. Dermatology. 21. 397-404 (1994)

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      1995 Annual Research Report
  • [Publications] Mizuno,K.: "Spontaneous production of interleukin-8 by human lung cancer cells and its augmentation by tumor necrosis factor alpha and interleukin-1 at protein and mRNA levels." Oncology. 51. 467-471 (1994)

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      1995 Annual Research Report
  • [Publications] Ito,A.: "Suppression of interleukin 8 production by progesterone in rabbit uterine cervix." Biochem. J.301. 183-186 (1994)

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      1995 Annual Research Report
  • [Publications] Kasahara,K.: "Selective expression of monocyte chemotactic and activating factor/monocyte chemoattractant protein 1 in human blood monocytes by mycobacterium tuberculosis." J. Infectious Dis.170. 1238-1247 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Murayama,T.: "Elevated cytokine levels in synovial fluid of rheumatoid arthritis correlates with the presence of cytomegalovirus genome." Autoimmunity. 17. 333-337 (1994)

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      1995 Annual Research Report
  • [Publications] Wada,T.: "Detection of urinary interleukin-8 in glomerular diseases." Kidney Int.46. 455-460 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakamoto,Y.: "Detection of the putative E2 protein of hepatitis C virus in human liver." J. Med. Virology. 42. 374-379 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Wada,T.: "Appearance of interleukin-8 in the Urine Predicts Acute rejection of Renal Transplantation." Nephrol. Dial. Transplant.9. 874-875 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Wada,T.: "Prevention of proteinuria in acute immune complex-mediated glomerulonephritis by an antibody against interleukin 8." J. Exp. Med.180. 1135-1140 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Murayama,T.: "Enhancement of human cytomegalovirus replication in human lung fibroblast cell line by interleukin 8." J. Virol.68. 7582-7584 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mukaida,N.: "Molecular mechanism of interleukin 8(IL-8)gene expression." J. Leukocyte Biology. 56. 554-558 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Harada,A.: "Essential involvement of interleukin 8(IL 8)in acute inflammation." J. Leukocyte Biology. 56. 559-564 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Oishi,K.: "Role of interleukin-8(IL-8)and an inhibitory effect of erythromycin on IL-8 release in the airways of patients with chronic airway diseases." Infect. Immun.62. 4145-4152 (1995)

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      1995 Annual Research Report
  • [Publications] Ko,Y.-C.: "Specific elevation of interleukin-8 levels in peritoneal dialysates of patients on continous ambulatory peritoneal dialysis with peritonitis." J. Clin. Pathol.48. 115-119 (1995)

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      1995 Annual Research Report
  • [Publications] Morohashi,H.: "Expression of both types of human interleukin 8(IL-8)receptors on mature neurtophils, monocyts, and CD16+lymphocytes." J. Leukocyte Biol.57. 180-187 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yano,S.: "Differential effects of anti-inflammatory cytokines(IL-4,IL-10,and IL-13)on tumorcidal and chemotactic properties of human monocytes induced by monocyte chmotactic and activating factor(MCAF)." J. Leukcyte Biol.57. 303-309 (1995)

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      1995 Annual Research Report
  • [Publications] Yasumoto,K.: "Molecular analysis of cytokine network involved in causing cachexia in colon 26 adenocarcinoma-bearing mice." Cancer Res.55. 921-927 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ishikawa,Y.: "Establishment of lipopolysaccharide-dependent nuclear factor-kB activation in a cell-free system." J. Biol. Chem.270. 4158-4164 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Watanabe,S.-i.: "Prevention of endotoxin shock by an antibody against leukocyte integrin b2 through inhibiting production and action of tumor necrosis factor." Intl. Immunol.7. 1037-1046 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hoshino,Y.: "Monocyte chemoattractant protein 1 stimukates tumor necrosis and recruitment of macrophages into tumor in tumo-bearing mude mice: Increased granulocyte/macrophage progenitors in murine bone marrow." Exp. Hematol.3. 1035-1039 (1995)

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      1995 Annual Research Report
  • [Publications] Sagawa,K.: "Production of IL-8 and the other cytokines by T cell clones established from the ocular fluid of patients with Behcet's disease." Ocular Immunol. Inflamm.3. 62-71 (1995)

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      1995 Annual Research Report
  • [Publications] Fujioka,N.: "Preparation of specific antibodies against murine interleukin 1 receptor antagonist(IL-1 ra)and the establishment of IL-1 ra as endogenous regulator of bacteria-induced fulminant hepatitis in mice." J. Leukocyte Biol.58. 90-98 (1995)

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      1995 Annual Research Report
  • [Publications] Hirose,K.: "Chemokine gene transfer into tumor cells reduced tumorigenicity in vivo mediated through neutroophilic infiltrate." Brit. J. Cancer.72. 708-714 (1995)

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      1995 Annual Research Report
  • [Publications] Sugiyama,Y.: "Chemokines in bronchoalveolar lavage fluid in summer-type hypersenitivity penumonitis." Eur. Respir. J.8. 1084-1090 (1995)

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      1995 Annual Research Report
  • [Publications] Ikeda,N.: "Prevention of bacterial induced hepatocelluar necrosis in rabbits by an antibody to leukocyte integrin b2 with concomitant inhibition of cytokine production." Infect. Immun.63. 4812-4817 (1995)

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      1995 Annual Research Report
  • [Publications] Nakashima,E.: "MCAF gene transfer enhances the metastatic capacity of mouse cachectic adenocarcinoma cell line." Pharmaceutical Res.12. 1598-1604 (1995)

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      1995 Annual Research Report
  • [Publications] Zhang,Y.: "TNFa as a physiological regulator of hematopoietic progenitor cells: Increase of early hematopoietic progenitor cells in TNF-R55-1-mice in vivo and potent inhibition of progenitor cell proliferation by TNFa in vitro." Blood. 6. 2930-2937 (1995)

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      1995 Annual Research Report
  • [Publications] Teranishi,Y.: "Increased spontaneous production of IL-8 in peripheral blood monocytes from the psoriatic patient: relation to focal infection and response to treatments." J. Dermatol. Science. 10. 8-15 (1995)

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      1995 Annual Research Report
  • [Publications] Larsen,C.G.: "The delayed-type hypersensitivity reaction is dependent on IL-8. Inhibition of a tuberculin skin reaction by an anti-IL-8 monoclonal antibody." J. Immunol.155. 2151-2157 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kuno,K.: "Identification of an IkBa-associated protein kinase in a human cell line and determination of its phosphorylation sites on IkBa." J. Biol. Chem.270. 27914-27919 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Xiu,Q.: "Bronchial hyperresposiveness and airway neutrophil accumulation induced by interleukin-8 and the effect of the thromboxane A2 antagonist S-1452 in guinea-pigs." Clin. Exp. Allergy.25. 51-59 (1995)

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      1995 Annual Research Report
  • [Publications] Yamamoto,N.: "Regulatory mechanisms for production of interferon-g and TNF by anti-tumor T cells or macrophages in the tumor-bearing state." J. Immunol.154. 2281-2289 (1995)

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      1995 Annual Research Report
  • [Publications] Jinquan,T.: "Recombinant human growth-regulated oncogene-a induces T lymphocyte chemotaxis." J. Immunol.155. 5359-5368 (1995)

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      1995 Annual Research Report
  • [Publications] Inoue,H.: "Expression of cytokines and adhesion molecules in hemodialysis-associated amyloidosis" Nephrol. Dialysis Transplant.(in press).

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      1995 Annual Research Report
  • [Publications] Wada,T.: "Monitoring urinary levels of monocyte chemotactic and activating factor(MCAF)reflects disease activity of lupus nephritis." Kidney Int.(in press).

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      1995 Annual Research Report
  • [Publications] Su,S-b.: "Rapid secretion of intracellularly pre-stored interleukin-8 from rabbit platelets upon the activation." J. Leukocyte Biol.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Mukaida,N.: "Novel insight into molecular mechanism of endotokxin shock: biochemical analysis of LPS receptor signaling in a cell-free system targeting NF-kB and regulation of cytokine production/action through inteann b2 in vivo." J. Leukocyte Biol.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Nielsen,B.W.: "Macrophage-Pathogene Interactions" Marcel Dekker, 11 (1994)

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      1995 Annual Research Report
  • [Publications] Harada,A.: "Ares-Sereno Symposia Publications" Challenges of Modern Medicine Vol.3, 6 (1994)

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      1995 Annual Research Report
  • [Publications] Matsushima,K.: "Proceeding of IIIrd International Mochida Memorial Symposium" The Mochida Memorial Foundation for Medical and Pharmaceutical Research, 8 (1994)

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      1995 Annual Research Report
  • [Publications] Okamoto,S.: "In the Chemokines Proceeding of IIIrd International Symposium of Chemotactic Cytokines" Plenum, 10 (1994)

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      1995 Annual Research Report
  • [Publications] Murakami,S.: "Viral Hepatitis and Liver Disease." 4 (1994)

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      1995 Annual Research Report
  • [Publications] Mukaida,N.: "In Leukocyte Typing V" Oxford University Press, 3 (1995)

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      1995 Annual Research Report
  • [Publications] Morohashi,H.: "In Leukocyte Typing V" Oxford University Press, 3 (1995)

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      1995 Annual Research Report
  • [Publications] Mukaida,N.: "Cytokines: interleukins and their receptors." Kluwer Academic Publishers(in press),

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      1995 Annual Research Report
  • [Publications] Harada,A.: "In Growth factor and cytokines in health and disease." JAI press(in press),

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      1995 Annual Research Report
  • [Publications] Harada,A.: "In ImmunoMethod on Chemokine" Academic press(in press),

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  • [Publications] Harada,A.: "The Roles of Chemokines in Disease" R. G. Landes Company(in press),

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      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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