| Project/Area Number |
07557073
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
内分泌・代謝学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
KITA Toru Kyoto University, Graduate School of Medicine, Professor, 医学研究科, 教授 (60161460)
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| Co-Investigator(Kenkyū-buntansha) |
ISHII Kenji Kyoto University, Graduate School of Medicine, Instructor, 医学研究科, 助手 (00212811)
WAKATSUKI Yoshio Kyoto University, Graduate School of Medicine, Lecturer, 医学研究科, 講師 (40220826)
KUME Noriaki Kyoto University, Graduate School of Medicine, Instructor, 医学研究科, 助手 (20252455)
YOKODE Masayuki Kyoto University, Graduate School of Medicine, Lecturer, 医学研究科, 講師 (20252447)
DOI Toshio Kyoto University, Graduate School of Medicine, Lecturer, 医学研究科, 講師 (60183498)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 1996: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1995: ¥10,000,000 (Direct Cost: ¥10,000,000)
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| Keywords | Cholesterol / Apo B-protein / MTP / VLDL / CHO-cell / Hepatocytes / HSP-70 / Transgenic mouse / LDL / LDL受容体 / HMG-CoA還元酵素阻害剤 / 家族性複合型高脂血症 / 家族性高コレステロール血症 / WHHLウサギ |
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| Research Abstract |
We found apolipoprotein B-100 (apo B) secretion is regulated in response to the change of intracellular cholesteryl ester contents by adding low density lipoprotein (LDL). LDL caused a significant dose-dependent increase in apo B secretion, because of preventing the degradation of apo-B protein itself. Immediately after apo B protein is translated from mRNA of apo B,microsomal triglyceride transfer protein (MTP) makes assembly with apo B protein. As a result, VLDL particles are formed. Therefore MTP plays an important role for regulating VLDL production and secretion. To investigate this regulation, we first cloned full length of cDNA for MTP,succeeded to transfect MTP cDNA to CHO cells and detected MTP protein. We are now doing following experiments.
1) To establish MTP overexpression system in rabbit hepatocytes.
2) To establish transgenic mouse which over express MTP cDNA using transferin promoter.
3) To analyze proteins which could regulate post-translational modification of apo-B protein, suchi as MTP,HDP-70 and unknown protein.
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