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Development of a Near-Field/Atomic-Force Microscope and its Application to Studies of Biological Membranes

Research Project

Project/Area Number 07558102
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field Cell biology
Research InstitutionThe University of Tokyo

Principal Investigator

KUSUMI Akihiro  The University of Tokyo, Graduate School of Arts and Sciences, Associate Professor, 大学院・総合文化研究科, 助教授 (50169992)

Co-Investigator(Kenkyū-buntansha) ATAKA Tatsuaki  Seiko Instruments, Inc., Basic Development Lab., Senior Scientist, 基礎技術研究所, 主管研究員
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1996: ¥6,500,000 (Direct Cost: ¥6,500,000)
Keywordsnear-field microscopy / near field / AFM / NSOM / atomic force microscope / plasma membrane / cytoskeleton / 近接場顕微鏡 / 原始間力顕微鏡 / 光学顕微鏡 / 生細胞 / 水中観察 / 分解能
Research Abstract

The scanning near-field optical microscopy imaging of speciments in liquid and of cultured cells in aqueous solutions is reported. A scanning near-field optical/atomic-force microscope (SNOM-AFM) was developed, in which the scanning of an optical-fiber probe cantilever over the specimen was controlled by noncontact mode AFM (dynamic mode AFM). This imaging mode reduces damage to the probe and soft specimens. The resonant frequency of the probe cantilever decreased 20% to approximately 14 kHz and the Q factor decreased by a factor of 8 to 30 in water, compared with these values in air, which was sufficient to perform SNOM-AFM imaging in liquid.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] A.Wisniewska: "Depth dependence of the perturbing effect of placing a bulky group (oxazolidine ring spin labels) in the membrane on the membrane phase" Biochim.Biophys.Acta. 1278. 68-72 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Muramatsu: "Scanning near-field optic/atomic force microscopy in liquids." Thin Solid Films. 273. 337-338 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Muramatsu: "Development of near-field optic/atomic-force microscope for biological materials in aqueous solutions." Ultramicroscopy. 61. 265-269 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Kusumi: "Cell surface organization by the membrane skeleton." Curr.Opinion Cell Biol.8. 566-574 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Kusumi and Y.Sako: "Cell surface organization by the membrane skeleton" Curr Opinion Cell Biol. 8. 566-574 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Muramatsu, N.Chiba, T.Umemoto, K.Homma, K.Nakajima, T.Ataka, S.Ohta, A.Kusumi, and M.Fujihira: "Development of near-field optic/atomic-force microscope for biological materials in aqueous solutions." Ultramicroscopy. 61. 265-269 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Wisniewska, Y.Nishimoto, J.S.Hyde, A.Kusumi, and W.K.Subczynski: "Depth dependence of the perturbing effect of placing a bulky group (oxazolidine ring spin labels) in the membrane on the membrane phase transition." Biochim.Biophys.Acta. 1278. 68-72 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Muramatsu, N.Chiba, K.Homma, K.Nakajima, T.Ataka, S.Ohta, A.Kusumi, and M.Fujihira: "Scanning near-field optic/atomic force microscopy in liquids." Thin Solid Films. 273. 337-338 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Wisniewska: "Depth dependence of the perturbing effect of placing a bulcy group(oxazolidine ring spin labels)in the membrane on the membrane phase transition." Biochim.Biophys.Acta. 1278. 68-72 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Muramatsu: "Scanning near-field optic/atomic force microscopy in liquids." Thin Solid Films. 273. 337-338 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Muramatsu: "Development of near-field optic/atomic-force microscope for biological materials in aqueous solutions." Ultramicroscopy. 61. 265-269 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] A.Kusumi: "Cell surface organization by the membrane skeleton." Curr.Opinion Cell Biol.8. 566-574 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y. Sako: "Barriers for lateral diffusion of transferrin receptor in plasma membrane as characterized by receptor dragging by laser tweexers" J. Cell Biol.129. 1559-1574 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Amisaki: "Error evaluation in the design of a special-purpose processor that calculates non-bonded forces in molecular dynamics simulations." J. Comp. Chem.16. 1120-1130 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H. Muramatsu: "Near-field optical microscopy in liquid." Appl. Phys. Lett.66. 3245-3247 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A. Wisniewska: "Pepth dependence of the perturbing effect of placing a bulky group(oxazolidine ring spin labels)in the membrane on the membrane phase transition" Biochim. Biophys. Acta. 1278. 68-72 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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