| Project/Area Number |
07558204
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
環境影響評価(含放射線生物学)
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| Research Institution | Kanazawa University |
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Principal Investigator |
ISHIGAKI Yasuhito (1996) Kanazawa Univ., Dept.of Pharm.Sci., Research Associate, 薬学部, 助手 (20232275)
二階堂 修 (1995) 金沢大学, 薬学部, 教授 (60019669)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUNAGA Tsukasa Kanazawa Univ., Dept of Pharm.Sci., Lecturer, 薬学部, 講師 (60192340)
SUZUKI Fumio Hiroshima Univ., RIRBM,Professor, 原爆放射能医学研究所, 教授 (10019672)
石垣 靖人 金沢大学, 薬学部, 教務職員 (20232275)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Biological UVB dosimeter / Solar light / pyrimidine dimer / Nitrocellulose filter / Risk assessment / Monoclonal antibody / DNA damage |
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| Research Abstract |
In this study, we tried the establishment and the characterization of the personal solar UV dosimetry system which is like afilm badge has been used for radiation monitoring. We used single strand DNA molecules on a small nitrocellulose membrane (2*4 cm) as a photoreceptor. This membrane was sealed up by a polyethylene filter with a silica gel that keeps humidity low. Various types of damage such as cyclobutane type pyrimidine dimer are induced in the DNA by solar UV exposure. After exposure to solar light, this membrane was treated with a cyclobutane type pyrimidine dimer specific monoclonal antibody ; TDM-2 and this was treated with the horseradish peroxidase conjugated second antibody. The colorization of diaminobendizine on the membrane by this enzyme was quantitated by an image analyzer. The amount of colorization was correlated lineary with irradiated UV dose by Oriel solar simulator whithin a limited dose range, over this dose range the colorization level remains relatively constant. However when we used a blue polyethylene filter that cuts UV penetration to the membrane, the linear correlation was extended past the total daily UV dose per day in Japan. Induced damage was kept stable on the membrane for at least 22.days at both 37ﾟC and 4ﾟC , This membrane kept it's ability to form cyclobutane pyrimidine dimer for more than 21 days when stored at both 37ﾟC and 4ﾟC.We observed no changes of the induction of cyclobutane type pyrimidine dimer in the temperature range range between 15ﾟC to 70ﾟC.
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