|Budget Amount *help
¥1,900,000 (Direct Cost : ¥1,900,000)
Fiscal Year 1996 : ¥800,000 (Direct Cost : ¥800,000)
Fiscal Year 1995 : ¥1,100,000 (Direct Cost : ¥1,100,000)
1) Localization of a G protein in the rat ependymal cilia Go and Gi belong to G proteins that transduce signals from the receptor in the cell membrane to the effectors such as ion channels and enzymes. It has been reported that the G protein present in the ependymal cilia facing the ventricular system of rat brains is Go (Peraldi et al., 1989). However, the present study supported by this grant has clearly shown that Gi2 is characteristically localized in the ciliary membrane of the ependymal cells. In addition, it was revealed that Gi2 was localized in the cilia of the oviduct and trachea, but not in the stereocilia of the ductus deferens. These findings suggest a important role for Gi2 in the ciliary functions in these tissues.
2) Localization of Heme oxygenase-2 (HO-2) in the rat olfactory system The odorants increase the cGMP levels in the olfactory cells. However, the addition of HO-2 inhibitor abolished such an increase in cGMP levels, suggesting that HO-2 may play a role in the olfactory system (Verma et al., 1993). This grant helped us to investigate the localization of HO-2 in the rat olfactory system, using anbibodies raised against a peptide sequence of HO-2. It was found that HO-2 was localized in a variety of cells in both the main and the accessory olfactory pathways, including the olfactory cells, sensory cells in the vomeronasal organ and many neurons in the olfactory bulb. This finding suggests an active involvement of HO-2 in the olfactory system.
2、嗅覚系におけるHeme oxygenase-2 (HO-2)の局在
嗅細胞に“におい"物質を加えるとcGMPレベルが上昇するが、HO-2阻害剤の存在下ではcGMPの上昇は認められない(Verma et al., 1993)。この事は、HO-2がにおいの情報伝達になんらかの役割を果している事を示している。そこで本研究では、HO-2のペプチドに対する抗体を作成して、ラット嗅覚系におけるHO-2の局在を免疫組織化学的に検索した。その結果、HO-2は嗅粘膜の嗅細胞と副嗅球系の鋤鼻器官の感覚細胞をはじめ嗅神経線維、鋤鼻神経、嗅球の僧帽細胞や糸球体周囲の神経細胞など嗅覚系の細胞に広く分布しており、においの情報伝達に重要な役割を果していることが示唆された。