ANTICOAGULANT ACTIVITY OF SULFATIDE AND ITS EFFECTS ON ARTERIOSCLEROSIS

Research Project

Project/Area Number	07670141
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	General medical chemistry
Research Institution	SHINSHU UNIVERSITY
Principal Investigator	HARA Atsushi SHINSHU UNIVERSITY,LECTURER, 医学部附属加齢適応研究センター, 講師 (70126697)
Co-Investigator(Kenkyū-buntansha)	UEMURA Kei-ichi SHINSHU UNIVERSITY,ASSOCIATE PROFESSOR, 医学部附属加齢適応研究センター, 助教授 (80012756) TAKETOMI Tamotsu SHINSHU UNIVERSITY,PROFESSOR, 医学部附属加齢適応研究センター, 教授 (30020704)
Project Period (FY)	1995 – 1997
Project Status	Completed (Fiscal Year 1997)
Budget Amount *help	¥2,200,000 (Direct Cost: ¥2,200,000) Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000) Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000) Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Keywords	SULFATIDE / ANTICOAGULANT / FIBRINOGEN / PROTHROMBIN TIME / THROMBIN / BLEEDING TIME / PLATELET / sulfdtide / anticodgulant / Prothrombin time / bleeding time / Pldtelet / Sulfatide / dnticoagulant / Platelet
Research Abstract	SULFATIDES (GALACTOSYLCERAMIDE I^3-SULFATE), WHICH ARE FOUND IN SERUM LIPOPROTEINS OF VARIOUS MAMMALS,EFFECTIVELY INCREASED PROTHROMBIN TIME (ANTICOAGULANT EFFECT) AND ALSO EFFECTIVELY PROLONGED BLEEDING TIME (ANTI-PLATELET EFFECT). WHEN EQUAL VOLUMES OF A HOMOGENEOUS MICELLAR SOLUTION OF SULFATIDE AND FIBRINOGEN IN PHOSPHATE BUFFERED SALINE WERE MIXED,AN INSOLUBLE COMPLEX PRECIPITATED.ANALYSIS OF THE PRECIPITATED COMPLEX SHOWED THAT THE MOLAR RATIO OF SULFATIDE TO FIBRINOGEN WAS ABOUT 400 : 1. THESE RESULTS INDICATE THAT THE SULFATIDE MICELLEBINDS TIGHTLY TO FIBRINOGEN AND THEREBY INTERFERES WITH BOTH FIBRIN GEL FORMATION (ANTICOAGULANT ACTIVITY) AND PLATELET FUNCTION.