|Budget Amount *help
¥2,300,000 (Direct Cost : ¥2,300,000)
Fiscal Year 1996 : ¥400,000 (Direct Cost : ¥400,000)
Fiscal Year 1995 : ¥1,900,000 (Direct Cost : ¥1,900,000)
We investigated histologically and immunohistochemicaly the differences between hepatitis associated bile duct damage in chronic hapetitis C and chronic non-supprative destructive cholangitis in primary biliary cirrhosis. Histologically, the former was associated with formation of lymphfollicles, in contrast, the latter was associated with eosinophilic infiltration and granulomatous reaction. Immunohistochemically, the expression profile of apomucins (MUC1, MUC2, MUC3 and MUC5/6) and cytokines (IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-8, IFN gamma, TNF alpha, TNF beta) showed differences and similarities respectively. The results suggested the different sequence in the two types of bile duct damage.
Recentry, we experinenced an autosomal recessive mutant mouse named aly/aly mouse that lacks systemic lymph nodes and also a variable lymphoid cell infiltration with lymph follicles formation in the portal tract, and variable damages in the intrahepatic biliary epithelial cells including pseudopyloric gland metaplasia and proliferative changes. Some of these lesions were reminiscent of primary biliary cirrhosis. In addition, extrahepatic bile duct and intrahepatic large bile duct contained acidophilic substance in their epithelial cytoplasm. Aly/aly mouse may be a good animal model to analyze the metabolism of the biliary substances, and immune-mediated bile duct damages may be produced in this model by immunological modulations. So we go on the investigation.