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Evaluation of tetrameric microsatellites for use in personal identification of Japanese

Research Project

Project/Area Number 07670484
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionNiigata University

Principal Investigator

YAMANOUCHI Haruo  Niigata Univ.School of Medicine Professor, 医学部, 教授 (30134919)

Co-Investigator(Kenkyū-buntansha) DEWA Koji  Niigata Univ.School of MedicineAssistant, 医学部, 助教授 (10197832)
NAITO Emiko  Niigata Univ.School of MedicineAssistant, 医学部, 助手 (80018811)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsMicrosatellite / STR / Personal Identification / PCR-based DNA typing / vWF / Haplotype / AR / Androgen recepter
Research Abstract

Polymorphic microsatellites (short tandem repeat : STR) are the genetic markers very useful in human identification. We previously reported that PCR-based DNA typing of two tetrameric STRs vWF-p and TH01 provided an accurate and rapid discrimination for personal identification of Japanese. In this study, two STR loci vWF-k (TCTA) n and AR (CAG) n (CAA) were evaluated.
(1) Typing for vWF-k of 460 unrelated Japanese was done. Nice alleles were observed. The heterozygosity was 0.798 and the polymorphism information content (PIC) was 0.765.
(2) The usefulness of a haplotype analysis of vWF-k and vWF-p loci in forensic investigations was examined, since a haplotype produces more variation than an allotype. These two tetrameric repeat loci are located within approximately 700 bp of intron 40 of the von Willebrand factor gene. Their polymorphisms were determined by PCR for DNA obtained from a total of 57 families and haplotypes were constructed on the basis of the family data. As many as 29 haplotypes were identified of 116 unrelated individuals included in the families. Its heterozygosity and polymorphism information content were 0.984 and 0.928, respectively. Among the 116 individuals, 72 were distinguished using the simply combined genotyping of vWF-p. On the other hand, 77 diplotypes were determined with the haplotype analysis. This suggests usefulness of the testing in personal discrimination, especially in cases haveing the family information. Moreover, this haplotype analysis has proved to be a powerful tool in forensic case studies.
(3) Typing for AR locus of 110 unrelated Japanese is under investigation.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 山内春夫: "DNA診断の法医学への応用" 医学のあゆみ. 174. 522-527 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hajime Fujita: "Three Short Tandem Repeat Loei in Japanese Population" Jpn J Legal Med. 49. 213-221 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 藤田 一: "日本人における3種のマイクロサテライト(GCG, vWF, TH)多型について" 「DNA多型」. 3. 124-126 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 内藤笑美子: "vWF遺伝子内におけるSTRのハプロタイプ(2座位)について" 「DNA多型」. 4. 93-95 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 出羽厚二: "vWF遺伝子内の2座位のSTRハプロタイプ分析-日本人頻度とその法医学的応用法-" 日本法医学雑誌. 50. 349-356 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Haruo Yamanouchi: "DNA diagnosis in legal medicine" IGAKU NO AYUMI. Vol.174. 522-527 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hajime Fujita: "Three short tandem repeat loci in Japanese population" Jpn J Legal Med. Vol.49. 213-221 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hajime Fujita: "Three polymorphic microsatellite markers (GCG,vWF,TH) in Japanese" DNA POLYMORPHISM. Vol.3. 124-126 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Emiko Naito: "Haplotype frequencies of the two STRs on HUMVWFA31" DNA POLYMORPHISM. Vol.4. 93-95 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Koji Dewa: "Haplotype analysis using two tetrameric loci within the vWF gene : Its frequency in Japanese and application in forensic medicine" Jpn J Legal Med. Vol.50. 349-356 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 山内春夫: "DNA診断の法医学への応用" 医学のあゆみ. 174. 522-527 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hajime Fujita: "Three Short Tandem Repeat Lociin Japanese Populatin" Jpn J Legal Med. 49. 213-221 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 藤田一: "日本人における3種のマイクロサテライト(GCG,vWF,TH)多型について" DNA多型. 3. 124-126 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 内藤笑美子: "vWF遺伝子内におけるSTRのハプロタイプ(2座位)について" DNA多型. 4. 93-95 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 出羽厚二: "vWF遺伝子内の2座位のSTRハプロタイプ分析-日本人頻度とその法医学的応用法-" 日本法医学雑誌. 50. 349-356 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 山内春夫: "DNA診断の法医学への応用" 医学のあゆみ. 174. 522-527 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hajime Fujita: "Three Short Tandem Repeat Loci in Japanese Population" Jpn J Legal Med. 49. 213-221 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 藤田一: "日本人における3種のマイクロサテライト(GCG,vWF,TH)多型について" DNA多型. 3. 124-126 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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