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Establishment of universal detection method for virus genome in sera from patients with acute liver damage

Research Project

Project/Area Number 07670620
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionThe Jikei University School of Medicine

Principal Investigator

FUJISE Kiyotaka  The Medical Department, The Jikei University School of Medicine Assistant Professor, 医学部, 助教授 (60057057)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsAcute liver damage / Virus genome / PCR method / Hepatitis non-A,B,C / Hepatitis G / Silent hepatitis B / ヘルペスウイルス
Research Abstract

Patients with acute liver damage, who were excluded drug induced, alcholic and autoimmune hepatitis and without serum virus markers of hepatitis A,B and C in acute phase, were studied as subjects in this project. Firstly to deny the existence of hepatitis B virus (HBV) and hepatitis C virus (HCV) in the sera obtained from these patients, nested polymerase chain reaction (PCR) for HBV-DNA at major S region and nested reverse transcription (RT) -PCR for HCV-RNA at 5'-noncoding region were performed. HCV gene was amplified from 4 of the 17 patients and they were diagnosed as acute hepatitis C,however HBV gene was not ampified none of them. Detection of virus genome by these PCR methods was performed in patients with acute hepatitis A and B as controls. It was comfirmed that the timing of sampling and the selection of efficient method for extraction of nucleic acids were important. Secondly to examine the participation of hepatitis G and silent hepatitis B without ordinary HBV markers in the sera from selected patients with acute hepatitis non-A,B,C including 2 fulminant hepatitis, nested RT-PCR for hepatitis G virus (HGV) -RNA at 5'-noncoding region and helicase region and nested PCR for HBV-DNA at precore region were performed. HBV gene was amplified from 8 of the 10 patients, however HGV gene was not ampified none of them. The original plan for the establishment of universal detection method for virus genome in sera from patients with acute liver damage has changed, but it has became clear that the silent HBV infection is the main cause of patients with acute hepatitis non-A,B,C.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 藤瀬清隆: "B型急性肝炎各種病型における変異ウイルスの関与" 肝臓. 36suppl(2). 67- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 内藤嘉彦: "A型急性肝炎におけるPCR法による血中ウイルスの検討" 感染症学雑誌. 70. 386-388 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 新谷稔: "いわゆる非A非B非C型散発性急性肝炎症例における原因ウイルスの検索と臨床経過" 日本消化器病学会雑誌. 93増刊(2). 268- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujise, K: "Hepatitis B virus variants in patients with acute hepatitis in whom various clinical forms develop." Acta Hepatol Jpn. 36-suppl (2). 67 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Naito, Y: "Detection of hepatitis A virus in serum by PCR method." J Jpn A Inf D. 70. 386-8 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Niiya, M: "The study of etiology and clinical course on patients with sporadic non-A,B,C acute viral hepatitis." Jpn J Gastroenterol. 93-suppl (2). 268 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 新谷稔: "いわゆる非A非B非C型散発性急性肝炎症例における原因ウイルスの検索と臨床経過" 日本消化器病学会雑誌. 93・増刊(2). 268 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 内藤嘉彦: "A型急性肝炎におけるPCR法による血中ウイルスの検討" 感染症学雑誌. 70(4). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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