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Immunological mechanisms of liver disturbance in patients with chronic hepatitis C.

Research Project

Project/Area Number 07670640
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionFukui Medical School (1996)
Tokyo Metropolitan Organization for Medical Research (1995)

Principal Investigator

YABU Koji  Fukui Medical School, Assistant, 医学部, 助手 (60240793)

Co-Investigator(Kenkyū-buntansha) 小原 道法  財団法人東京都臨床医学総合研究所, 微生物研究部門, 研究員 (10250218)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsChronic hepatitis C / recombinant antigens of HCV / cellular immunological responses / antigen-spesific proliferative responses
Research Abstract

The assumption is that the reason why patients with chronic hepatitis C can not eradicate hepatitis C virus (HCV) in natural course is that immunological defense mechanisms against HCV does not work enough in such patients. It is generally accepted that cellular immunological defense of the host play important roles in exclusion of the virus persistently infecting. It has not been fully examined in cellular immunological aspects to eradicate HCV.The purpose of this study is to investigate how T lymphocytes of the patients respond in vitro to HCV antigens derived from recombinant HCV proteins or glycoproteins. Recombinant antigens to the genetic area coding HCV core (Pc), HCV envelopes of E1 and E2/NS1 (Ac-E1 and Ac-NS-1) and HCV non coding region of NS3 (Pn3) were made by recombined baculovirus or E.coli. The lymphocyte proliferative responses to these recombinant HCV antigens were tested using lymphocytes separated from peripheral blood lymphocyte of the patients with CH-C.
NS-3 and core-antigen specific responses in all patients groups were significantly higher that in the healthy control groups. E-1 and E2/NS1-antigen-specific responses in the patients group with ALT levels exceeding 100 IU/L were significantly higher than those in other patient groups. Histological diagnosis was not correlated to the intensity of the core-and NS3-specific responses. E1-and E2/NS1-antigens induced significantly elevated responses in patients with chronic active hepatitis and liver cirrhosis compared with results in the healthy control group and in patients with chronic presistent hepatitis. In conclusion, the significantly elevated responses to core-and NS3-antigens may be related to HCV infection and such responses to E1-and E2/NS1-antigens could be related to the severity and activity of the disease.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report

Research Products

(3 results)

All Other

All Publications (3 results)

  • [Publications] Hiromitsu Mori: "Lymphocyte proliferative responses to recombinant hepatitis C virus antigens in patients with chronic hepatitis C" J of Gastroenterol and Hepatol. 11. 697-704 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiromitsu Mori, et al.: "Lymphocyte proliferative responses to recombinant hepatitis C virus antigens in patients with chronic hepatitis C." J of Gastroenterol.and Hepatol. 11. 697-704 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiromitsu Mori: "Lymphocyte proliferative responses to recombinant hepatitis C virus antigens in patients with chronic hepatitis C" J of Gastroenterol and Hepatol. 11. 697-704 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1995-03-31   Modified: 2016-04-21  

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