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Molecularepidemiology of Parainfluenza virus type 4 infection

Research Project

Project/Area Number 07670684
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionSuzuka University of Medical Scinece and Technology

Principal Investigator

KOMADA Hiroshi  Suzuka University of Medical Science and Technology, Department of Clinical Nutrition, Associate Professor, 保健衛生学部, 助教授 (10144247)

Co-Investigator(Kenkyū-buntansha) TURUDOME Masato  Mie University School of Medicine Department of Microbiology, Associate Professo, 医学部, 助教授 (50159042)
ITO Yasuhiko  Mie University School of Medicine Department of Microbiology, Professor (00022872)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsparainfluenza virus type 4 / expressed cell / HN protein / ELISA / virus neutralization / パラインフルエンザウイルス4型 / F蛋白 / HN蛋白 / シークエンス
Research Abstract

About 250 human sera were collected, and they were analyzed by enzyme linked-immunosorbent assay (ELISA). We found that about 20 sera were positive for human parainfluenza type 4 virus (HPIV-4). The same sera had neutralization activity against HPIV-4. However, it was impossible to distinguish whether the sera inhibited HPIV-4A or 4B growth. To analyze whether the infection was caused by HPIV-4A or 4B,we established L929 cell lines which express HN proteins of HPIV-4A or 4B,because there was a big antigenic difference between HPIV-4A and 4B HN proteins. The identification is now in progress.
To elucidate the cause of the antigenic difference between HPIV-4A and 4B,3 mutated clones of 4B HN gene were constructed, and we established L929 cell lines which express the mutated HN proteins of HPIV-4B.The antigenicity was analyzed by flow cytometry using monoclonal antibodies against HPIV-4, and it was indicated that the sugar binding sites may in part contribute to the antigenic difference between HPIV-4A an 4B HN proteins.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report

Research Products

(25 results)

All Other

All Publications (25 results)

  • [Publications] Machiko Nishio: "Interaciton between nucleocapsid protein (NP) and phosphoprotein (P) of human parainfluenza virus type 2 : one of the two NP binding sites on P is essential for granule formation" Journal of General Virology. 77. 2457-2463 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Noriko Watanabe: "Identification of the sequences responsible for nuclear targeting of the V protein of human parainfluenza virus type 2" Journal of General Virology. 77. 327-338 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shigeru Suga: "Intracellular localization of antigens recognized by anti-vimentin monoclonal autibodies (mAbs) : cross-reactivities of anti-vimentin mAbs with other cellular components" European Journal of Cell Biology. 70. 84-91 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Noriko Watanabe: "Binding of the V proteins to the nucleocapsid proteins of human parainfluenza type 2 virus" Medical Microbiology and Immunology. 185. 89-94 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shinji Ohgimoto: "Regulation of human immunodificiency virus gp 160-mediated cell fusion by antibodies against fusion regulatory protein 1" Journal of General Virology. 77. 2747-2756 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiroshi Komada: "Early calcium signaling and calcium requirements for the IL-2 receptor expression and IL-2 production in stimulated lymphocyles" Cellular Immunology. 173. 215-220 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Noriko watanabe, Hiroshi Komada, Yasuhiko Ito et al.: "Identification the sequence responsible for nuclear targeting of the V protein of human parainfuruenza virus type 2." Journal of General Virology. 77. 327-338 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Xiaojuan Mao, Hiroshi Komada, Yasuhiko Ito et al.: "Characterization of Bango virus proteins by using monoclonal antibodies" Avian Disease. 40. 150-157 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shigeru Suga, Hiroshi komada, Yasuhiko Ito et al.: "Intracellular localization of antigens recognized by anti-vimentin monoclonal antibodies (mAbs) : cross-reactivities of anti-vimentin mAbs with other cellular components" European Jounal of Cell Biology. 70. 84-91 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Noriko Watanabe, Hiroshi Komada, Yasuhiko Ito et al.: "Binding of the V proteins to the nucleocapsid proteins of human parainfluenza type 2 virus." Med.Microbiol.Immunol.185. 89-94 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Machiko Nishio, Hiroshi Komada, Yasuhiko Ito et al.: "Interaction between nucleocapsid protein (NP) and phosphoprptein (P) of human parainfluenza virus type 2 : one of the two NP binding sites on P is essential for granule formation" Journal of General Virology. 77. 2457-2463 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiroshi Komada, Hiroshi Nakabayashi, Kosaku Izutsu et al.: "Early calcium signaling and calcium requirements for the IL-2 receptor expression and IL-2 production in stimulated lymphocytes" Cellular Immunology. 173. 215-220 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shinji Ohgimoto, Hiroshi Komada, Yasuhiko Ito et al.: "Regulation of human immunodeficiency virus gp 160-mediated cell fusion by antbodies against fusion regulatory protein 1." Journal of General Virology. 77. 2747-2757 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Machiko Nishio: "Interaction between nucleocapsid protein (NP) and phosphoprotein (P) of human parainfluenza virus type 2 : one of the two NP binding sites on P is essential for granule formation" Journal of General Virology. 77. 2457-2463 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Noriko Watanabe: "Identification of the sequences responsible for nuclear targeting of the V protein of human parainfluenza virus type 2" Journal of General Virology. 77. 327-338 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shigeru Suga: "Intracellular localization of antigens recognized by anti-vimentin monoclonal antibodies (mAbs) : cross reactivities of anti-vimentin mAbs with other cellular components" European Journal of Cell Biology. 70. 84-91 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Noriko Watanabe: "Binding of the V proteins to the nucleocapsid proteins of human parainfluenza type 2 virus" Medical Microbiology and Immunology. 185. 89-94 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shinji Ohgimoto: "Regulation of human immunodificiency virus gp 160-mediated cell fusion by antibodies against fusion regulatory protein 1" Journal of General Virology. 77. 2747-2756 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hiroshi Komada: "Early calcium signaling and calcium requirements for the IL-2 receptor expression and IL-2 production in stimulated lymphocytes" Cellular Immunology. 173. 215-220 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tetsuya Yuasa: "A cell fusion-inhibiting monoclonal antibody, binds to the presumed storlk domain of the human parainfluenza Type2 virus henagglutlnin-neuraminidase protein" Virology. 206. 1117-1125 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 伊藤守弘: "ヒト型パラインフルエンザ4B型ウイルスF蛋白の遺伝子解析および発現" 鈴鹿医療科学技術大学紀要. 2. 125-132 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shinji Ohgimoto: "Molecular Characterization of fusion regulatury protein-1 that induces multinacleated giant cell formation of monocytes and HIVqp160-mediated cell fusion" Journal of Immunology. 155. 3585-3592 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masato Tsurudome: "Identification of regions on the hemagglutinin-neuraminidase protein of human parainfluenza virus type2 emportant for promoting cell fusion" Virology. 213. 190-203 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shigeru. Suga: "Identification of fusion regulatory protein(FRP)-1/4F2 related molecules" Cell Structure and Function. 20. 473-483 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiroshi Komada: "Sequence analysis of human parainfluenza virus type 4A and type 4B fusion proteins" Journal of General Virology. 76. 3205-3210 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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