Pathogenesis of HTLV-I positive polymyositis

• Project/Area Number: 07670721
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: Kagoshima University
• Principal Investigator: HIGUCHI Itsuro University Hospital Kagoshima University Assistant Professor, 医学部・附属病院, 講師 (80183573)
• Co-Investigator(Kenkyū-buntansha): IZUMO Shuji Faculty of Medicine Kagoshima University Professor, 医学部, 教授 (30143811)
• Project Period (FY): 1995 – 1996
• Project Status: Completed(Fiscal Year 1996)
• Budget Amount: ¥2,600,000 (Direct Cost : ¥2,600,000); Fiscal Year 1996 : ¥1,000,000 (Direct Cost : ¥1,000,000); Fiscal Year 1995 : ¥1,600,000 (Direct Cost : ¥1,600,000)
• Keywords: HTLV-I / Polymyositis / PCR in situ hybridization / HAM / PCR / in situ hybridization / in situ PCR / Polymyositis / ATL

Research Abstract

Patients with polymyositis (PM) in Kagoshima, Japan exhibit seropositivity to human T-cell lymphotropic virus type I (HTLV-I), which is higher than in the general population. A direct HTLV-I infection of muscle fibers was previously reported by in situ hybridization (ISH) in a patient dually infected with HIV and HTLV-I.While using the same technique of ISH,we could not detect HTLV-I in patients with PM infected with HTLV-I alone. We obtained biopsied muscle specimens from 5 HTLV-I positive PM patients, 5 HTLV-I negative PM patients, an autopsied muscle specimen from a HAM patient, and a lymph node from a patient with adult T-cell leukemia (ATL) as a positive control. Employing in situ PCR,we found the HTLV-I provirus in some of the CD4-positive infiltrating cells in HTLV-I-positive PM but not within the muscle fibers. Among HTLV-I-positive PM,we performed double staining of immunohistochemistry for CD4 or macrophage and in situ PCR for HTLV-I provirus on the same sections to determine the exact cell types of HTLV-I containing cells. We found HTLV-I in CD4-positive cells but not in macrophages. This finding suggests that most of the HTLV-I containing CD4-positive cells are not macrophages but lymphocytes.

Research Products

• [Publications] I,Higuchi et al.: "The main HTLV-I harboring cells in the muscles I viral carriers with polymyosltis are not macrophages but CD41 Iymphocytes." Acta Neuropathologica. 92. 358-361 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] I,Higuchi et al.: "Detection of HTLV-I provirus by in situ polymerase chain reaction in mononuclear iflammatory cells in skeletal muscle of viral carriers with polymyositis." Muscle & Nerve. 18. 854-858 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] I,Higuchi et al.: "In situ polymerase chain reaction detection of HTLV-I provirusand expression of the p53 tumor suppressor gene in infiltrating cells in skeletal muscle from a patient with adult T cell leukemia." Acta Neuropathologica. 90. 323-327 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] I.Higuchi, K.Hashimoto et al.: "The main HTLV-I harboring cells in the muscles of viral carriers with polymyositis are not macrophages but CD4-positive lymphocytes." Acta Neuropathol. 92. 358-361 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] I.Higuchi, K.Hashimoto et al.: "Detection of HTLV-I provirus by in situ polymerase chain reaction in mononuclear inflammatory cells in skeletal muscle of viral carriers with polymyositis." Muscle & Nerve. 18. 854-858 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] I.Higuchi, K.Hashimoto et al.: "In situ polymerase chain reaction detection of HTLV-I provirus and expression of the p53 tumor suppressor gene in infiltrating cells in skeletal muscle from a patient with adult T cell leukemia." Acta Neuropathol. 90. 323-327 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] I.Higuchi et al.: "The main HTLV-I harboring cells in the muscles I viral carriers with polymyositis are not macrophages but CD4_+ lymphocyles." Acta Neuropathologica. 92. 358-361 (1996)
• [Publications] I.Higuchi et al.: "Detection of HTLV-I provirus by in situ polymerase chain reaction in mononuclear iflammatory cells in skeletal muscle of viral carriers with polymyositis." Nuscle & Nerve. 18. 854-858 (1995)
• [Publications] I.Higuchi et al.: "In situ polymerase chain reaction detection of HTLV-I provirusand expression of the p53 tumor suppressor gene in infiltrating cells in skeletal muscle from a patient with adult T cell leukemia." Acta Neuropathologica. 90. 323-327 (1995)
• [Publications] Itsuro Higuchi et al.: "Detection of HTLV-I provirus by in situ plymerae chain reaction in mononuclear inflammatory cells in skeletal muscle of viral carriers with polymyositis." Muscle & Nerve. 18. 854-858 (1995)
• [Publications] Itsuro Higuchi et al.: "In situ polymerase chain reaction detection of HTLV-I provirus and expression of the p53 tumor suppressorgene in infiltrating cells in skeletal muscle from a patient with adult T cell leukemia" Acta Neuropathologica. 90. 323-327 (1995)