| Project/Area Number |
07670806
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Showa University |
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Principal Investigator |
KATAGIRI Takashi School of Medicine, Showa University, Professor, 医学部, 教授 (90102293)
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| Co-Investigator(Kenkyū-buntansha) |
GESHI Eiichi School of Medicine, Showa University, Assistant Professor, 医学部, 講師 (50192050)
KONNO Noburu School of Medicine, Showa University, Assistant Professor, 医学部, 講師 (50215472)
YANAGISHITA Toshikuni School of Medicine, Showa University, Assistant Professor, 医学部, 講師 (70183671)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Ischemia-Reperfusion / Free radial / ESR / Cerium chloride / Catalase / Sarcoprasmic reticulum / Mitochondria / ACE inhibitor / Ca-ATPase / Hydrogen peroxide / Superoxide dismutase / 再潅流障害 / フリーラジカル / SOD / catalase / working heart / cerium chloride |
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| Research Abstract |
Object :
We studied the role of free radicals on the reperfusion injury in the various experimental models of ischemic heart.
Results :
1. We could detect the free radicals with electron spin resonance (ESR) method using spin trapping agent, POBN,in the rat and dog models of ischemia-reperfused heart from 30 sec to 5 min after reperfusion.
2. Ca^<++>-ATPase activity of sarcoplasmic reticulum (SR) and its composing protein was earlier degradated in the ischemia-reperfused heart than ischemia-nonreperfused heart in the rat and dog models.
3. In the perfused rat heart and isolated myocyte, free radical generations from mitochondria and endothelial cell were detected visually using Cerium chloride, reactant to the hydrogenperoxide, with ultrastructural observation.
4. In the stunned heart of the dog model (15 min ischemia followed by 60 min reperfusion), contractile function and its SR function were more improved with treatment of superoxide dismutase (SOD) and ACE-inhibitor (alacepril).
Conclusion :
Free radial generation was one of the initial factors of the reperfusion injury.
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